Ca2+signal生物体内每一个细胞都是一个生产和输出分子(如胰岛素、抗体、酶等)的工厂.这些分子在细胞内都是以“细胞囊泡”的形式传递的.James•E•Rothman,Randy•W•Schekman &Thomas•C•Südhof等三位科学家因发现了这些“细胞囊泡”是如何被在正确的时间输运至正确地点的分子机制之谜而获得2013年...
解析Ca2+离子在糖代谢和脂代谢中的作用能够为理解棉花纤维快速伸长阶段细胞膜和细胞壁的动态变化提供有意义的参考。 近期,西南大学农学与生物科技学院棉花分子育种团队张正圣教授课题组完成的题为“Comparative transcriptome and lipidome rev...
在三个Ca2+离子的作用下，水母发光蛋白分子将腔肠素-h底物氧化为腔肠酰胺，释放二氧化碳并在469nm处产生具有峰值的闪光，通过VICTOR Nivo检测。由于GPCR激活后细胞内Ca2+浓度增加且相应的闪光产生速度非常快，因此该检测需要极快速的测量响应。VICTOR Nivo读板仪的分液器可以满足这一需求，它可以在进样后0.1秒从微孔...
This suggests a connection between the Ca2 + signal and CaM as its transducer, controlling the regulation of this enzyme that is required for the functionality of active SFKs in the cell. However, no proof of action of this phosphorylation on the activity of NMT was provided. The concentration...
These mutant mice also have nuclear PC aggregates that are not required for SCA1 pathogenesis, since in a transgenic model where the nuclear localization signal is deleted, similar pathological and clinical phenotypes to ATXN1poly82Q mice, were observed. In support of this, Sca1154Q knock-in ...
Intracellular Ca2+signaling is considered to be important for multiple astrocyte functions in neural circuits. However, mice devoid of inositol triphosphate type 2 receptors (IP3R2) reportedly lack all astrocyte Ca2+signaling, but display no neuronal or neurovascular deficits, implying that astrocyte Ca2...
We show that the local Ca2+ signal produced by a single opening of an LCC, named a ‘Ca2+ sparklet’, can trigger about 4–6 ryanodine receptors to generate a Ca2+ spark. The coupling between LCCs and ryanodine receptors is stochastic, as judged by the exponential distribution of the ...
Caffeine caused no drop in the aequorin signal but did cause a drop in the chlortetracycline fluorescence. These results are consistent with the idea that aequorin reports cytoplasmic intracellular calcium concentration ([Ca2+]i), chlortetracycline reports stored calcium, and fura-2 reports a mixed ...
We show that the local Ca2+ signal produced by a single opening of an LCC, named a 'Ca2+ sparklet', can trigger about 4-6 ryanodine receptors to generate a Ca2+ spark. The coupling between LCCs and ryanodine receptors is stochastic, as judged by the exponential distribution of the ...
Karlstad J, Sun Y and Singh BB. Ca(2+) signal- ing: an outlook on the characterization of Ca(2+) channels and their importance in cel- lular functions. Adv Exp Med Biol 2012; 740: 143-57.Karlstad J, Sun Y, Singh BB. Ca(2+) signaling: an outlook on the charac- terization of...