In an in vitro bystander killing assay, proliferation of Trop-2-negative cells was potently inhibited by addition/transfer of culture medium of BAT8008-treated Trop-2-positive cells, but not that of BAT8008-treated Trop-2-negative cells, indicating the bystander killing effect of the released ...
thymidine kinase(TK) gene. In the presence of theprodrugganciclovir, TK expression kills not only the TK-transfected cells but also the nearby untransfected cells [116]. The gap junction that mediates the intercellular communication appears to be responsible for the TK-induced bystander killing [...
either for indicated times (2, 4, 6 or 8 h for caspase-8 assay) of for 24 h (cell lysis/killing assay). For these studies, multiple technical replicates but at least two biological replicates were carried out. For caspase-8 assays, the reagents were added to the cultures at ...
To determine whether BA-CTLs can directly lyse NKG2D-ligand-expressing target cells, we used the VITAL in vitro killing assay (Hermans et al., 2004). We found that OT-I BA-CTLs could kill RMA-Rae1-expressing cells (but not RMA cells), albeit less efficiently than SIINFEKL pulsed RMA...
Given previously published work on listeria monocytogenes (LM) infection as a model for CD8 bystander killing17, we used an LM-ova protection assay with the adoptive transfer of VM cells isolated from antigen-specific (OT1) or antigen-irrelevant (gBT-1) donors (Fig. 5a). VM cells typically...
By using cervical cancer cells and differentiated macrophages, we demonstrate that MMC induces the expression of FasL via upregulation of PPARγ in both cell types (effector cells) in vitro, but it failed to induce bystander killing in cervical cancer cells. This effect was p...
(Fig.3b) and was associated with reduced levels of infection at day 7 postviral infection (pvi) as determined by immunostaining on lung tissue (Fig.3c) and plaque assay (Fig.3e). We next measured the levels of infection over time using MuHV-4-luc recombinant virus for live imaging of ...
Bystander cell killing spreading from endothelial to tumor cells in a three-dimensional multicellular nodule model after Escherichia coli nitroreductase ge... Tumor cells are elusive targets for standard anticancer chemotherapy due to their heterogeneity and genetic instability. On the other hand, prolifera...
This might explain the observed TK-hAMSCs migration towards the tumor tissue for an efficient tumor killing effect. However, the bystander effect of TK-hAMSCs located in the tumor limits cannot be discarded, as it might also be responsible of the cancer cell killing death in some extent. The...
Cell killing at 48 hours was significantly decreased when CytoB was added to the top chamber containing NV1066 alone (30 versus 11%; P = 0.0016) or both NV1066 and GCV (18 versus 71%; P = 0.002; Figure 3). These findings indicate that abrogation of TNTs reduced transfer of activated...