We performed single-cell RNA sequencing (scRNA-seq) of dental cells from embryonic day 14.5 (E14.5) mice to understand the heterogeneity of developing tooth germ cells. Computational analyses including gene regulatory network (GRN) assessment were conducted. We validated our findings using immunohistoch...
Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3′ cDNA libraries for dozens of sa
RNA samples were aligned to the RefSeq build 73 transcriptome using Bowtie2 v.2.2.6 and quantified using RSEM v.1.2.2528. Gene expression was quantified using Salmon v.0.9.1 with hg19 reference from Ensembl v.75. Mutation Sequencing data were processed by the bcbio-nextgen pipeline. Briefly,...
RNA sequencingSingle cellSingle nucleusTranscriptomeParkinson’s diseaseNeurodegenerative diseaseParkinson's disease (PD) is a chronic and progressive neurodegenerative disease leading to motor dysfunction and, in some cases, dementia. Transcriptome analysis is one promising approach for characterizing PD and ...
Traditional high-throughput omics studies just focused on the macroscopic analysis of the common features of mixed cell components. Emerging single-cell RNA sequencing (scRNA-seq) and spatial transcriptome sequencing (ST-seq) enable the acquisition of multilayer molecular information with unprecedented reso...
Illumina TruSight One Expanded Sequencing Panel (36 samples) FC-141-2007 102240 Illumina NeoPrep? Proof of Concept SP-801-1004 36500 Illumina LENS TISSUE 15005277 940 Illumina ASSY. TARGET, FILTER, O.D. 2.0 15006413 6680 Illumina ASSY. TARGET, FILTER, O.D. 0.5 15006761 7300 ...
Single-cell technologies have proven ideal for studying immune heterogeneity and can be used to identify specific pathogenic cell populations, which is key for advancing drug discovery [6]. The single-cell RNA sequencing (scRNA-seq) datasets, data analysis tools, and various applications continue to...
RNA-sequencing has become a standard tool for analyzing gene activity in bulk samples and at the single-cell level. By increasing sample sizes and cell counts, this technique can uncover substantial information about cellular transcriptional states. Beyo
Finally, most of the true fusions were present by more than one RNAseq read. This provides the basis for automatic annotation of 3′RTK fusions using FFPE RNAseq profiles. Keywords: fusion oncogene; receptor tyrosine kinase; RNA sequencing; RNAseq; FFPE; tumor molecular diagnostics; clinical ...