Bulk RNA-sequencing pipeline流程(含代码) 对于Bulk RNA-seq测序(用于比较转录组学,如不同物种的同种组织样本,也就是我们常说的常规转录组测序,注意和单细胞测序区分),我们常用的分析流程有很多,之前的文章也有介绍。偶然在github上发现一个总结的很完整的分析流程和大家分享。 我们依照作者的思路来进行: 使用NCBI ...
今天给大家介绍的 就是这篇发表在《Frontiers in Genetics》上题为"Integrated analysis of bulk and single-cell RNA sequencing reveals the interaction of PKP1 and tumor-infiltrating B cells and their therapeutic potential for nasopharyngeal carcinoma" 的文章,IF= 4.8 。作者在文章的Acknowledgement也是对我们...
对illumina数据进行处理,利用 RNA-Seq 发现新的 RNA 变体和剪接位点,或量化 mRNA 以进行基因表达分析等。对两组或多组样本的转录组数据,通过差异表达分析和对所发现的差异表达基因集合进行功能富集分析以推断生物学功能。 数据准备: 数据下载: Humangenome(GRCh38/hg3):Index of /goldenPath/hg38/chromosomes (ucs...
早!今天小编和大家分享一篇22年11月发表在Frontiers in Immunology(IF:8.786)杂志的文章《Identification and validation of a signature based on macrophage cell marker genes to predict recurrent miscarriage by integrated analysis of sin...
英文标题:Development of a CD8+ T cell associated signature for predicting the prognosis and immunological characteristics of gastric cancer by integrating single-cell and bulk RNA-sequencing 期刊:Sci Rep. 2024 Feb 24;14(1):4524. 影响因子:2区4.6 DOI: 10.1038/s41598-024-54273-9. 研究领域:免...
title:Integrated analysis of single-cell and bulk RNA sequencing data reveals a pan-cancer stemness signature predicting immunotherapy response author: author time: 2022.04 journal: genome medicine(IF = 15) Objective: 癌症干细胞可能是 ICI 耐药的潜在原因,但缺乏直接的临床证据。
今天分享的科研文章探讨了通过结合单细胞和bulk RNA测序技术，以预测复发性流产（RM）的机制，特别是通过分析巨噬细胞细胞标记基因的表达模式。研究团队从GEO数据库获取了相关数据集，通过无监督聚类分析注释细胞类型，并筛选出与RM相关的差异表达巨噬细胞相关基因。经过一系列统计分析，包括LASSO、随机森林和...
Integrated analysis of single-cell and bulk RNA sequencing data reveals immune-related lncRNA-mRNA prognostic signature in triple-negative breast cancer. Genes Dis. 2023 May 10;11(2):571-574. doi: 10.1016/j.gendis.2023.04.006. PMID: 37692501; PMCID: PMC10491898....
Cost-efficient library generation by early barcoding has been central in propelling single-cell RNA sequencing. Here, we optimize and validate prime-seq, an early barcoding bulk RNA-seq method. We show that it performs equivalently to TruSeq, a standard
RNA-sequencing (RNA-seq) has become an increasingly cost-effective technique for molecular profiling and immune characterization of tumors. In the past decade, many computational tools have been developed to characterize tumor immunity from gene expression data. However, the analysis of large-scale RNA...