早!今天小编和大家分享一篇22年11月发表在Frontiers in Immunology(IF:8.786)杂志的文章《Identification and validation of a signature based on macrophage cell marker genes to predict recurrent miscarriage by integrated analysis of s
RNA-sequencing (RNA-seq) has become an increasingly cost-effective technique for molecular profiling and immune characterization of tumors. In the past decade, many computational tools have been developed to characterize tumor immunity from gene expression data. However, the analysis of large-scale RNA...
Bulk RNA-sequencing pipeline流程(含代码) 对于Bulk RNA-seq测序(用于比较转录组学,如不同物种的同种组织样本,也就是我们常说的常规转录组测序,注意和单细胞测序区分),我们常用的分析流程有很多,之前的文章也有介绍。偶然在github上发现一个总结的很完整的分析流程和大家分享。 我们依照作者的思路来进行: 使用NCBI ...
今天给大家介绍的 就是这篇发表在《Frontiers in Genetics》上题为"Integrated analysis of bulk and single-cell RNA sequencing reveals the interaction of PKP1 and tumor-infiltrating B cells and their therapeutic potential for nasopharyngeal carcinoma" 的文章,IF= 4.8 。作者在文章的Acknowledgement也是对我们...
题目:Integrative Analysis of Bulk and Single-Cell RNA Sequencing Data Reveals Cell Types Involved in Heart Failure期刊:Front Bioeng Biotechnol.(Q1 IF:5.7) 日期:05/January/2022 DOI:https://doi.org/10.3389/fbioe.2021.779225 心力衰竭(HF)是一种慢性进行性综合症,死亡率很高。HF是一种严重的心功能障...
RNA sequencing (RNAseq) can reveal gene fusions, splicing variants, mutations/indels in addition to differential gene expression, thus providing a more complete genetic picture than DNA sequencing. This most widely used technology in genomics tool box has evolved from classic bulk RNA sequencing (RNA...
Google第一个就是官网介绍,可以立马理解Iso-Seq的字面意思了. Iso-Seq 就是 isoform sequencing,中文就是同源异构体测序,其实也是一种 RNA 测序技术. The challeng真核转录组(denovo/resequencing)及案例分析 参考: 转录组文章的常规套路 文章解读:<Science>小麦转录组研究文章 转录组数据饱和度评估方法 Paper这个...
文章标题:Integrative analysis of single‑cell and bulk RNA‑sequencing data revealed T cell marker genes based molecular sub‑types and a prognostic signature in lung adenocarcinoma发表时间:2024-01-10 发表杂志:Scientific Reports 影响因子:4.997 在线阅读链接: https://www.nature.com/articles/s41598...
对illumina数据进行处理,利用 RNA-Seq 发现新的 RNA 变体和剪接位点,或量化 mRNA 以进行基因表达分析等。对两组或多组样本的转录组数据,通过差异表达分析和对所发现的差异表达基因集合进行功能富集分析以推断生物学功能。 数据准备: 数据下载: Human genome(GRCh38/hg3):Index of /goldenPath/hg38/chromosomes (...