All the bioinformatics processing and analysis for plasma and saliva cfDNA used in W Lab. Created by Irene Choi. Install all the required packages, softwares, and tools! NOTE: You only need to do this once. Hoffman2 has most dependencies already preinstalled. Follow the steps below to install...
Tumor DNA only a small percentage of the total cfDNA Sample degrades rapidly, so storage and handling is critical RNA sequencing RNA from blood, fresh-frozen biopsy, FFPE Fine Needle Aspirates Core Needle Biopsies Single cells Total RNA (500 ng–2 ...
Circulating Free DNA 1 A research approach for the detection of somatic mutations at 0.5% frequency from cfDNA and cTc DNA using a multiplex sequencing assay targeting 2000 tumor mutationsDumitru BrinzaDownload Tuesday, Apr 21, 2015, 8:00 AM–12:00 PM ...
燃石医学与广东省人民医院吴一龙教授团队合作,共同开展了一项回顾性研究,旨在探索脑脊液cfDNA指导EGFR突变NSCLC脑膜转移患者奥希替尼耐药后续治疗疗效。 查看更多 全球首个 | 廖宁教授团队破译HER-2低表达乳腺癌临床与分子特征 发布日期:2023-02-20 燃石医学联合廖宁教授团队回顾分析了来自广东省人民医院的523例乳腺癌患者...
癌症诊断也在从肿瘤组织活检向液体活检偏移。不同于从肿瘤上切片提取DNA,癌症液体活检从循环肿瘤细胞(CTCs)或者患者血液中游离细胞中提取游离DNA(cfDNA)。就目前来看,由于提供了许多解刨和细胞结构的信息,肿瘤组织活检暂时不会消失,但是许多液体活检的研究人员表示,液体活检具有更多功能性。
TMAmpSeq 特有的扩增技术,能最大程度保证文库的特异性,右图是利用VAHTSTMAmpSeq Cancer HotSpot Panel(Vazyme #NA102) 建库.NGS 下机数据统计结果。从结果上看,相同样本的on-target 比例重复性好,一些极端样本(如FFPE、cfDNA) 的on-target 比例跟普通基因组DNA 的是一样的。
占全球测序市场份额80%的IIIumina在中国的临床肿瘤战略合作伙伴,与IIIumina启动Miseq dx测序仪联合临床实验 分子诊断解决方案 燃石医学将在中国开发基于Agilent SureSelect 靶向序列捕获系统的肿瘤基因检测,为中国肿瘤患者提供更多高品质的基于二代基因测序的分子诊断解决方案 学术合作 搭建分享、讨论真实世界精准诊疗...
Burning Rock’s proprietary blood-based circulating cell-free DNA (cfDNA) test for pan-cancer early detection is based on its ELSA-seq technology for the detection of methylation profiles. Through four years of development, Burning Rock’s ELSA-seq has demonstrated signs of potential effectiveness,...