The interaction between horse liver alcohol dehydrogenase and Reactive blue 2 immobilized on Sepharose CL-6B was measured by zonal chromatography. Each protein molecule was retained by a single immobilized dye using a Blue-Sepharose column containing a total of 1.38 mM dye. However, the protein was...
产品名称: 现货|Blue Sepharose 6 Fast Flow|Blue Sepharose 6FF|Blue-琼脂糖凝胶 6FF Blue-琼脂糖凝胶 6FF .0 订购此产品 供应商: GE 规格: 25ml分 目录价: 1270 库存状态: 现货 CAS编号: 应用范围: 种属来源: 相关信息: Blue Sepharose 6 Fast Flow BioProcess Medium: Yes Ligand: Cibacron Bl...
Column i.d.:7 mm 流速1< 4 ml/min 压力最大值(操作时填充床压力)5 bar [0.5 MPa] (70 psi) 储存:4 至 30°C,20% 乙醇 CytivaGE17041201 HiTrap Blue HP预填充预装柱,5 × 1 ml,1 × 5 ml,产品说明: HiTrap Blue HP 预填充有 Blue Sepharose High Performance,可捕获多种生物分子,包括:白蛋...
In contrast, Blue Sepharose affinity chromatography could efficiently separate free 80G from the semipurified conjugate because the conjugate was bound to the column by its gelonin moiety while free 80G was not in buffer containing NaCl of a particular concentration range. However, a small amount ...
(ATP: 3-phospho-d-glycerate 1-phosphotransferase, EC 2.7.2.3) from Escherichia coli AHU 1520 was purified until electrophoretically homogeneous by a single procedure, using a linear gradient elution of 2,3-bisphosphoglycerate between 0 and 0.5 mM on a blue dextran-Sepharose 4B column ...
Intriguingly, reversing the two proteins (Sepharose-bound GAPDH vs. free tubulin), resulted in the lack of a tubulin inhibition of GAPDH activity even at a 7 μM tubulin concentration. This suggested that the GAPDH tubulin binding was blocked as a function of GAPDH immobilization on the ...
Protein purification was conducted using an affinity column of Blue Sepharose resin (GE Healthcare), which specifically binds to proteins in the dehydrogenase/reductase family, according to the manufacturer’s instructions. The protein homogeneity and concen- ...
Cibacron Blue 3GA was immobilized on Sepharose CL-6B to obtain a highly substituted dye-ligand adsorbent which dye concentration was 17.4渭mol dye per gram... LZ He,YR Gan,Y Sun - 《Bioprocess Engineering》 被引量: 118发表: 1997年 Spectral changes induced in Cibacron Blue F3GA by salts, ...
The soluble fraction was obtained by centrifugation and loaded on a glutathione-Sepharose column equilibrated with PBS. Contaminating proteins were washed out with PBS, and TTR was digested out of the resin with thrombin (Calbiochem) at room temperature for up to 24 h. TTR-containing fractions ...
The bound protein was eluted in HBS containing 0.1% (w/v) LMNG, 0.01 mM TCEP (pH 7.0), and 250 mM imidazole (pH 8.0) and further purified over an in-house anti-FLAG M1 affinity Sepharose column as follows. The eluate was supplemented with 2 mM CaCl2 before loading over the anti-...