苯基-琼脂糖phenyl-sepharose疏水亲和层析
Improved purification of human liver alkaline phosphatase by phenyl-Sepharose column chromatography. Clin Chim Acta 1988;171:317- 23.Hirabayashi, Y., T. Nakao, M. Matsumoto, K. Obata, and S. Ando. 1988. Improved method for large-scale purification of brain gan- gliosides by Q-sepharose ...
Pressure/Flow Specification: Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm Storage Conditions: 4 to 30°C, 20% Ethanol Chemical Stability: Stable in commonly used aqueous buffers - 1 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene ...
HiLoad Phenyl Sepharose High Performance is available in two sizes, 16- or 26-mm diameter, both with a bed height of 10 cm and giving column volumes of 20 ml and 53 ml, respectively. In addition to the builtin benefits of high-resolution, high capacity preparative separations, HiLoad ...
To evaluate the contribution of high carbohydrate-containing fractions to the allergen content of Alternaria , samples of an Alternaria extract were bound to a phenyl Sepharose column and eluted with Tris buffer containing 4, 2, and 1 mol/L of NaCl and then distilled water. The fractions were ...
Pressure/Flow Specification: Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm Storage Conditions: 4 to 30°C, 20% Ethanol Chemical Stability: Stable in commonly used aqueous buffers - 1 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene ...
Observation of two protein bands in column elutions has also been report...Lopez F, Lobasso S, Colella M, Agostiano A, Corcelli A (1999) Light-dependent and bio- chemical properties of two different bands of bacteriorhodopsin isolated on phenyl- sepharose CL-4B. Photochem Photobiol 69:599...
pH Stability Cleaning-in-Place (CIP) 2-14 Pressure/Flow Specification Base matrix: 100-200 cm/h, 300 kPa, BioPilot 60/600 column, bed height 30 cm Storage Conditions 4 to 30°C, 20% Ethanol Chemical Stability Stable in commonly used aqueous buffers - 1 M NaOH, 1 M Acetic acid, 30...