Pressure/Flow Specification: 400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm Exclusion Limit [Mr] [Globular Proteins]: 4 x 106 Storage Conditions: 4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 ...
The chromatographic process was run at different pH range from pH 4 to pH 8 using 24 ml column. All major whey proteins were recovered in the elution fraction at pH 4 and pH 4.5. No protein was bound at pH 7 and pH 8. The best whey fractionation from 2 ml whey feed was achieved ...
Always read and follow the relevant column instruction manual carefully. Sepharose Fast Flow ion exchangers are easy to pack since their rigidity allows the use of high flow rates, see figure 1. Recommended bed heights are 10 to 15 cm. The different packing methods that are suitable for ...
p. 9 2. Column packing guidelines Sepharose Fast Flow ion exchangers CM, DEAE and Q are supplied in suspension in 20% ethanol as standard shipping solution. SP Sepharose Fast Flow is supplied in suspension in 20% ethanol and 0.2 M sodium acetate as standard. Large pack sizes of the media...
Nostoc sp. crude extract was treated with rivanol and then applied to a column of aminohexyl–Sepharose where C-phycoerythrin-II- and C-phycocyanin-containing fractions were eluted with 9.0 mM and 0.1 M phosphate buffer (pH 7.2), respectively. Both fractions were precipitated with 2.4 M ammonium...