HiTrap SP FF is prepacked with SP Sepharose Fast Flow, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions. Convenient and affordable for fast, easy ion exchange separations either alone or connected in series. ...
Benzamidine Sepharose 4 Fast Flow ( high sub ) HiTrap Benzamidine FF ( high sub )Benzamidine, Hitrap
17072910 17-0729-10 SP SEPHAROSE FAST FLOW, 25 ML 17079001 17-0790-01 OCTYL-SEPHAROSE CL-4B 200 ML 17081001 17-0810-01 PHENYL-SEPHAROSE CL-4B 200 M 17094602 17-0946-02 OCTYL SEPHAROSE 4 FAST FLOW 20 17094610 17-0946-10 OCTYL SEPHAROSE FAST FLOW, 25 17096001 17-0960-01 BUTYL SEPHAROSE...
Buffers: 0.05 M Tris, (+ 2 M NaCl in the elution buffer), pH 7.5 (Q and DEAE), 0.1 M acetate, (+ 2 M NaCl in the elution buffer), pH 5.0 (SP and CM). ANX Sepharose 4 Fast Flow (high sub): Sample was applied at 300 cm/h until 10% breakthrough. Column: 1.6 × 13 cm...
11 Note: If a P1-pump is used a max flow rate of 1–3 ml/min can be run on a HiTrap 1 ml column packed with Sepharose High Performance media. 4. Optimization If sample composition is unknown, a simple screening test with the aid of a syringe or pump can be performed to optimize ...
2 1. Description Media properties ™ SP Sepharose High Performance and Q Sepharose High Performance are strong cation and strong anion exchangers respectively. Both remain charged and maintain high capacity over broad pH ranges. The functional groups are coupled to the matrix via chemically stable ...
HiTrap™SPFF,HiTrapCMFF,HiTrapQFF,HiTrapDEAEFF,andHiTrap ANXFF(highsub)areprepacked1mland5ml,readytousecationand anionexchangeHiTrapcolumnsformethodscouting,group separations,sampleconcentrationandsampleclean-upofcharged biomolecules.Thespecialdesignofthecolumn,togetherwithamodern ...
HiTrap SP FF is prepacked with SP Sepharose Fast Flow, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions. Convenient and affordable for fast, easy ion exchange separations either alone or connected in series. The indust...
High flow rates and good scale-up potential. Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient. Predictable scale-up Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties an...