弱阴的结合强度比强阴弱,缓冲液只比等电点高0.5-1个pH单位的时候,用弱阴相当于不能结合。每一...
METHODS: A high polar fraction and its sub fractions were isolated from ‘Banlangen’ with dry column flash chromatography. 方法:用快速低压干柱柱层析法从板蓝根中分离出一个高极性流分及其亚流分 ;用过硫酸铵 /N,N ,N’,N’-四甲基乙二胺 ( AP- TEMED)体系检测氧自由基的清除率。 2. METHODS...
Diethylaminoethyl Sepharose (DEAE-Sepharose) microcolumn for enrichment of glycopeptidesAmino acids/peptidesBiological samplesMass spectrometry/ICP-MSN-Glycosylation is one of the most prevalent protein post-translational modifications and is involved in many biological processes, such as protein folding, ...
Virus purification using superheated buffer in place (SBIP) for packed bed chromatography Download EN Use of benzyl alcohol as a shipping and storage solution for chromatography media Download EN Column efficiency testing Download 需要更多帮助? 联系我们,我们的专家随时可以帮助您...
关键词:龙牙百合;多糖;DEAE乙ephaase Fast Flow;纯化 Puritcation technology of Lilium brownii mar.vimdulum polysacchariZes by DEAE胡eeharose Fast Flow column chromatography CHEN Xiao-meng1,LIU Cheng-mei2 (1.Shanghai Trade School,Shanghai200092,China;2・State Key Laboratory of Food Scieecc ...
28988948 XK 26/20 column 17018002 试剂DEAE-SEPHADEX A-50 17005001 葡聚糖凝胶G75试剂 17002001 SEPHADEX G-15 试剂 17061801 快速亲和琼脂糖G(分离蛋白质) 17040403 HITRAP PROTENIN G HP 17071910 试剂CM SEPHAROSE FAST FLOW 17018001 阳离子葡聚糖A50试剂 100 G 17524802 HISTRAP HP5X5ML 17133001 3-*硅...
column. Pooled fractions of activity are separated on a DEAE-cellulose column and eluted at 80mM NaCl. Final separation is by a diaminobutane-Sepharose column eluted at 40% saturation with ammonium sulfate. Hirschet al.[6]separated Ape2 activity (Leu↓NHNap and Lys↓NHNap specific) with a ...
Pressure/Flow Specification 300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm Exclusion limit (Mr) (globular proteins) ~4 × 10⁶ Storage 4 to 30°C, 20% Ethanol Chemical stability5 Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochlor...
Pressure/Flow Specification300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm Exclusion limit (Mr) (globular proteins)~4 × 10⁶ Storage4 to 30°C, 20% Ethanol Chemical stability5Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70%...
据说跟截面积有关,我直径1.5的柱子,高40cm,柱体积大概70ml,然后填料是DEAE-sepharose FF,洗脱时...