As a result, the PAXgene protocol was optimized to include off-column DNase treatments, which yielded high-quality RNA suitable for gene expression studies. Furthermore, the results suggest that RNA isolation with PAXgene is advantageous compared to traditional extraction methods for RT-PCR analysis ...
Optimization of a protocol for extraction of Plasmodium falciparum RNA from infected whole blood samples for use in DNA microarrays. Afr J Biotechnol. 2008;7:1461-7.Jaffu Chilongola,S. B. a. E. M."Optimization of a protocol for extraction of Plasmodium falciparum RNA from infected whole ...
between patients and controls in the RNA and imaging blocks of data while controlling for age, gender and the smoking status. When we examined the functional imaging block of data, we observed a significant effect of the factor of interest (e.g. Group,Pr( > F) = 0.015) whereas ...
RNA extraction from blood was performed using the QIAamp RNA blood mini kit (Qiagen, Carlsbad, CA) and RNA extractions for brain samples were carried out using the Qiagen AllPrep DNA/RNA/Protein mini kit (Qiagen, Carlsbad, CA), both according to the manufacturer’s protocol. RNA concentratio...
requiredinthisprotocol. Optional:RNaseAmaybeusedtodigestRNAduringtheprocedure.RNaseAis notprovidedintheDNeasyBloodTissueKit(see“CopurificationofRNA”, page19). Thingstodobeforestarting BufferALmayformaprecipitateuponstorage.Ifnecessary,warmto56°Cuntil theprecipitatehasfullydissolved. BufferAW1andBufferAW2areda...
Blood lead levels were determined according to the protocol developed by Koresková-Sysalová [24]. In both groups of animals the lead concentrations in whole blood samples after 30 days of treatment were measured in duplicate by atomic absorption spectrometry (Model AAS5 EA with graphite furnace...
RNA extraction and quality control The extraction of total RNA was done with the mirVana miRNA Isolation Kit (Thermo Fisher Scientific, Waltham, MA, USA). After centrifugation, the total RNA extraction protocol started with adding a chaotropic denaturing solution disrupting the cell samples immediately...
Simple Modifications to Standard TRIzol® Protocol Allow High-Yield RNA Extraction from Cells on Resorbable Materials RNA ExtractionTRIzolAcid Guanidium Thiocyanate - Phenol - Chloroform ExtractionTRI ReagentTRIsureResorbable bioceramics are attractive for medical applications such... J Tsz,L Yan,WH Tsang...
After centrifugation, the total RNA extraction protocol started with adding a chaotropic denaturing solution disrupting the cell samples immediately, followed by an acidic phenol: chloroform extraction. Ethanol was then added to the samples, and the solution was passed through a filter cartridge ...
RNA extraction and QC The samples were thawed overnight at 4°C before RNA extraction the following day. Total RNA was extracted using the Tempus 6-port RNA Isolation Kit Protocol with kit reagents and consumables in a 6100 Nucleic Acid Prep Station according to manufacturer’s instructions, inc...