As a result, the PAXgene protocol was optimized to include off-column DNase treatments, which yielded high-quality RNA suitable for gene expression studies. Furthermore, the results suggest that RNA isolation with PAXgene is advantageous compared to traditional extraction methods for RT-PCR analysis ...
Optimization of a protocol for extraction of Plasmodium falciparum RNA from infected whole blood samples for use in DNA microarrays. Afr J Biotechnol. 2008;7:1461-7.Chilongola J, Balthazary S, Mbugi E. Optimization of a protocol for extraction of Plasmodium falciparum RNA from infected whole ...
The extraction of total RNA was done with the mirVana miRNA Isolation Kit (Thermo Fisher Scientific, Waltham, MA, USA). After centrifugation, the total RNA extraction protocol started with adding a chaotropic denaturing solution disrupting the cell samples immediately, followed by an acidic phenol:ch...
S Florczak,K Kempka,Karolina Hoderna,... 被引量: 0发表: 2017年 Optimization of a Protocol for The Extraction of DNA from Human Blood for the Isolation and Expression of the Human Gene CtsK, in Bacterial Expression Syst... Cathepsin K, encoded by human gene CtsK is a major enzyme invol...
Simple Modifications to Standard TRIzol® Protocol Allow High-Yield RNA Extraction from Cells on Resorbable Materials RNA ExtractionTRIzolAcid Guanidium Thiocyanate - Phenol - Chloroform ExtractionTRI ReagentTRIsureResorbable bioceramics are attractive for medical applications such... J Tsz,L Yan,WH Tsang...
Ovarian function declines significantly as females enter middle-age, but the mechanisms underlying this decline remain unclear. Here, we utilize whole-organ imaging to observe a notable decrease in ovarian blood vessel (oBV) density and angiogenesis inte
Whole blood RNA signatures have been tested as biomarkers for TB, but have failed to meet the World Health Organization’s (WHO) optimal target product profiles (TPP). Here, we use RNA sequencing and machine-learning to investigate the utility of plasma cell-free RNA (cfRNA) as a host-...
We report a highly sensitive and rapid method for the extraction and detection of HIV-1 DNA in infant blood samples stored on filter papers. Because this rapid protocol does not involve steps for the removal of potential inhibitors of the PCR, the highest sensitivity is achieved by testing the...
Optimized protocol for the extraction of RNA and DNA from frozen whole blood sample stored in a single EDTA tube ArticleOpen access23 August 2021 Impact of 12-month cryopreservation on endogenous DNA damage in whole blood and isolated mononuclear cells evaluated by the comet assay ...
and A.D.S. The cell-free microbial DNA extraction protocol was originally designed and refined by C.C., S.F., S.M.-M., and A.D.S. The original version of the manuscript was written by G.D.P., A.D.S., S.P.P., and R.K. All authors contributed to the final version of ...