Comparison of mRNA extraction from whole blood (WB) and dried blood spots (DBS).Blauenfeldt, ThomasHeyckendorf, JanGraff Jensen, SidseLange, ChristophDrabe, CamillaS. Hermansen, Thomasde Thurah, LenaLillebaek, TroelsEugenOlsen, JesperSeersholm, Niels...
mRNA Extraction and Enrichment via Coated Plates The most distinctive mRNA purification method takes advantage of specially treated plates in conjunction with optimized reagents to both lyse and enrich mRNA from cells, tissue, blood, or purified total RNA. The Invi...
Total RNA was extracted from cells using a Total RNA Extraction Kit (R6812-01HP, Omega, United States). 1000 ng cDNA was synthesized with the aid of a cDNA synthesis kit (RR036A, Takara, Japan), diluted in 200 μL of ddH2O. A total of 10 μL of the qRT-PCR mix, consisting of...
Vaccine-induced T-cell responses were evaluated using T-cell proliferation assays and IFN-γ ELISpot. In the T-cell proliferation assay, T-cells from pre- and post-vaccination blood samples of the patients were incubated with either transfected DCs (tDCs) or non-transfected DCs (nDCs) and the...
a blood sample is drawn from the oncological patient to be used for autologous peripheral blood mononuclear cells (PBMC) extraction. These PBMCs are treated with differentiation factors to obtain immature DCs. Then, these immature DCs are treated with a cocktail of cytokines to produce mature antig...
2.2. Total RNA extraction from tissue specimen Formalin-fixed Paraffin Embedded tissue (FFPE) sections of 6–8 μ thickness were taken using tissue microtome. 4 to 5 μ thick paraffin sections were transferred into 1.5 mL labelled microcentrifuge tubes. The samples were deparaffinized and the RNA...
RNA extraction for RT-qPCR Sample processing was performed using established protocols.33 RNA from organ samples was extracted using the NuceloMag VET Kit (Macherey-Nagel, Düren, Germany) and the Biosprint 96 platform (QIAGEN, Hilden, Germany). Real-time RT-qPCR quantified viral RNA with the ...
Isoform sequencing (Iso-Seq) uses long-read technology to produce highly accurate full-length reads of mRNA transcripts. Visualization of individual mRNA molecules can reveal new details of transcript variation within understudied portions of mRNA, such as the 5′ untranslated region (UTR). ...
Fig. 5. Correlation of duodenal sHFE expression levels with peripheral blood iron metabolism biomarkers and duodenal HEPH expression. Duodenum biopsies from dyspepsia patients were used for RNA extraction and the expression levels of sHFE and HEPH transcripts assessed by RT-qPCR using HPRT1 as a no...
The onset of obsessive-compulsive disorder (OCD) involves the interaction of heritability and environment. The aim of this study is to identify the global messenger RNA (mRNA) expressed in peripheral blood from 30 patients with OCD and 30 paired healthy