A BGC prediction system identifies candidate biosynthetic gene clusters (BGCs) within genomes using machine-learned models, such as a shallow neural network and recurrent neural network (RNN). A set of domains within a genome sequence are identified, each domain corresponds to a set of domain ...
Numerous examples of biosynthetic gene clusters (BGCs), including for compounds of agricultural and medicinal importance, have now been discovered in plant genomes. However, little is known about how these complex traits are assembled and diversified. Here, we examine a large number of variants ...
a comparative analysis of the strains' biosynthetic potential was conducted in relation to other strains. The findings revealed that NRPS constituted the predominant class of biosynthetic gene clusters (BGCs) in bothXenorhabdusandPhotorhabdusgenomes, comprising 51% of the total BGCs. ...
Terpenoids, critical components of human medicine, are the largest family of natural products. Fungi are an important source of terpenoids, but many of the corresponding biosynthetic gene clusters (BGCs) are silent in laboratory conditions. Strategies su
We first investigated the secondary metabolic potential of marine prokaryotes by analyzing the diversity and novelty of the biosynthetic gene clusters (BGCs) in 7541 prokaryotic genomes from cultivated and single cells, along with 26,363 newly assembled medium-to-high-quality genomes from marine environ...
BGCs with a rich source of MIBiG-compliant data. Moreover, this annotation effort offered an ideal opportunity to evaluate the MIBiG standard in practice on a diverse range of BGCs. Hence, we carefully mined the literature to obtain a set of 1,170 experimentally characterized gene clusters: ...
Streptomyces bacteria are known for their prolific production of secondary metabolites, many of which have been widely used in human medicine, agriculture and animal health. To guide the effective prioritization of specific biosynthetic gene clusters (BG
Here we report an efficient CRISPR–Cas9 knock-in strategy to activate silent biosynthetic gene clusters (BGCs) in streptomycetes. We applied this one-step strategy to activate multiple BGCs of different classes in five Streptomyces species and triggered the production of unique metabolites, including ...
Microorganisms provide us with a large number of biosynthetic gene clusters (BGCs) that produce bioactive secondary metabolites [ 1 ]. However, many of these are being produced by microorganisms that grow slowly, or are even unculturable at laboratory conditions [ 2 ]. Moreover, the majority of...
biosynthetic gene clusters (BGCs). A BGC is a group of two or more different classes of non-homologous genes which are physically clustered, transcriptionally linked, and functionally related1,2,3,4,5,6. Over 30 plant BGCs have been functionally validated to date7since the discovery of the ...