Breakthrough in efficient cloning and activation of large cryptic biosynthetic gene clusters from high GC actinobacteriadoi:10.1016/j.synbio.2022.06.006Jun-Yi.WangFrontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical ...
124Citations 43Altmetric Metrics Abstract Bacteria contain an immense untapped trove of novel secondary metabolites in the form of ‘silent’ biosynthetic gene clusters (BGCs). These can be identified bioinformatically but are not expressed under normal laboratory growth conditions. Methods to access the...
Fig. 3: Differing patterns of diversity among gene families and biosynthetic gene clusters in hybridA. latusgenomes. AAmong a total of 10,078 orthologous groups of genes, 7485 are categorized as core (present in 100% of isolates;N = 22) and 2593 as accessory (present in <100% of i...
Chromatin level regulation of biosynthetic gene clusters Nat Chem Biol, 5 (2009), pp. 462-464 CrossrefView in ScopusGoogle Scholar [8] R.B. Williams, J.C. Henrikson, A.R. Hoover, A.E. Lee, R.H. Cichewicz Epigenetic remodeling of the fungal secondary metabolome Org Biomol Chem, 6 ...
Identifying gene clusters is a powerful tool for small molecule discovery and provides insight into the ecology and evolution of organisms. Current detection algorithms focus on canonical 'core' biosynthetic functions many gene clusters encode, while overlooking uncommon or unknown cluster classes...
CRYPTIC METABOLITES AND METHOD FOR ACTIVATING SILENT BIOSYNTHETIC GENE CLUSTERS IN DIVERSE MICROORGANISMSMicroorganisms are prolific producers of natural products, a group of molecules that make up the majority of drugs approved by the FDA in the past 35 years. After decades of mining, the low-...
CRYPTIC METABOLITES AND METHOD FOR ACTIVATING SILENT BIOSYNTHETIC GENE CLUSTERS IN DIVERSE MICROORGANISMSMicroorganisms are prolific producers of natural products, a group of molecules that make up the majority of drugs approved by the FDA in the past 35 years. After decades of mining, the low-...
CRYPTIC METABOLITES AND METHOD FOR ACTIVATING SILENT BIOSYNTHETIC GENE CLUSTERS IN DIVERSE MICROORGANISMSMicroorganisms are prolific producers of natural products, a group of molecules that make up the majority of drugs approved by the FDA in the past 35 years. After decades of mining, the low-...
Bacteria harbor an immense reservoir of potentially new and therapeutic small molecules in the form of "silent" biosynthetic gene clusters. These clusters can be identified bioinformatically but are at best sparingly expressed under normal laboratory growth conditions; their products are therefore not ...
CRYPTIC METABOLITES AND METHOD FOR ACTIVATING SILENT BIOSYNTHETIC GENE CLUSTERS IN ACTINOMYCETE BACTERIADisclosed is a high-throughput transcriptional assay format in Actinomycete bacteria, and spp. in particular, that leverages eGFP, inserted both at a neutral site and inside the biosynthetic cluster of ...