Bacterial genomes contain large reservoirs of biosynthetic gene clusters (BGCs) that are predicted to encode unexplored natural products. Heterologous expression of previously unstudied BGCs should facilitate the discovery of additional therapeutically relevant bioactive molecules from bacterial culture collections...
In order to identify distinctive or divergent gene clusters associated with potential antimicrobial synthesis, a comparative analysis of the strains' biosynthetic potential was conducted in relation to other strains. The findings revealed that NRPS constituted the predominant class of biosynthetic gene cluste...
In microbes, natural products are synthesized by groups of colocalized genes called biosynthetic gene clusters (BGCs). With advances in high-throughput sequencing, there has been an increase of complete microbial isolate genomes and metagenomes, from which ...
However, advanced techniques like genome sequencing have recently demonstrated that the chemical diversity captured by the traditional culture-based approaches is only the tip of the iceberg, and there are still a significant number of silent or cryptic biosynthetic gene clusters (BGCs), which are ...
Corrigendum: Chromatin-level regulation of biosynthetic gene clusters Loss-of-function Aspergillus nidulans CclA, a Bre2 ortholog involved in histone H3 lysine 4 methylation, activated the expression of cryptic secondary metabolite clusters in A. nidulans. One new cluster generated monodictyphenone, emo...
Interestingly, the rdm cluster contains a five-gene regulation system RdmACDEF, which is different from other reported polyene gene clusters. In vivo experiments demonstrated the XRE family regulator RdmA and the PAS/LuxR family regulator RdmF function in negative and positive manner, respectively. ...
We first investigated the secondary metabolic potential of marine prokaryotes by analyzing the diversity and novelty of the biosynthetic gene clusters (BGCs) in 7541 prokaryotic genomes from cultivated and single cells, along with 26,363 newly assembled medium-to-high-quality genomes from marine environ...
Biosynthetic gene clusters (BGCs) typically include a gene encoding a core biosynthetic enzyme, most commonly a nonribosomal peptide synthetase (NRPS), polyketide synthase (PKS), or terpene cyclase, that is responsible for the first metabolic step in product synthesis (11). Additionally, BGCs ...
Modification of the chromatin landscape in fungal secondary metabolite clusters allows for a simple technological means to express silent fungal secondary metabolite gene clusters.This is a preview of subscription content, log in via an institution to check access. ...
Fungi are an important source of terpenoids, but many of the corresponding biosynthetic gene clusters (BGCs) are silent in laboratory conditions. Strategies such as homologous activation and heterologous expression were usually used to active a single cluster, making them low efficiency. Here we ...