Biosynthetic gene cluster to produce a complex polyketidesPolyketide synthase with a module of a total of 15, non-ribosomal peptide synthetase with one module, and I describe the composed polyketide synthase complex from cytochrome p450 hydroxylase. I also provides a method of Streptomyces species new...
In addition, a probable L-glutamate-2,3-aminomutase, Strop_2771 (69% identity to IdnL4), is also encoded in the salinilactam biosynthetic gene cluster, indicating that these two types of enzymes are responsible for 3-aminobutyrate formation to supply a pathway-specific b-amino acid for the ...
we uncovered one unidentifiedt1pks-1gene clusters and one unidentifiednrpks-3gene cluster (Fig.4C). To distinguish these clusters, we have named them as “plu00736” and “plu00747,” respectively. The absence of matches in the MIBiG, Known ClusterBlast, and our in-house databases...
In microbes, natural products are synthesized by groups of colocalized genes called biosynthetic gene clusters (BGCs). With advances in high-throughput sequencing, there has been an increase of complete microbial isolate genomes and metagenomes, from which ...
The Biosynthetic Gene Cluster of Pantocin A Provides Insights into Biosynthesis and a Tool for Screening ( This work was supported by the National Institutes of Health (National Cancer Institute, R01-CA24487). ) doi:10.1002/ange.200351054Angewandte ChemieMi JinSandra A. I....
In this study, we found that a putative dehydrogenase gene (AK103462) and two functionally unknown P-450 genes (CYP99A2 and CYP99A3) form a chitin oligosaccharide elicitor- and UV-inducible gene cluster, together with OsKS4 and OsCyc1, the diterpene cyclase genes involved in momilactone ...
cluster probably originated from gene duplication and chromosomal translocation of native homologs of Bx genes.An ancient Bx cluster that included additional Bx genes(e.g.,Bx6)is presumed to have been present in ancestral Panicoideae.The ancient Bx cluster was putatively gained by the Triticeae ...
stockiae strain Xsto SBRx11.1 genomes, we uncovered one unidentified t1pks-1 gene clusters and one unidentified nrpks-3 gene cluster (Fig. 4C). To distin- guish these clusters, we have named them as "plu00736" and "plu00747," respectively. The absence of matches in the MIBiG, Known ...
Therefore, in this study, we aimed to quantitatively evaluate the biosynthetic chemical space of marine prokaryotes by comparing the numbers of biosynthetic gene cluster families (GCFs) and the molecular families (MFs) of reported marine prokaryotic natural products. Since the core peptides encoded by...
23). By comparing the Stachelhaus codes of each A domain in the cmx gene cluster to a manually constructed database of codes with known substrates, the following peptide sequence was predicted for 2: Thr1-Hpg2-Thr3-Gly4-Leu5-Pro6-Leu7-Gly8-Tyr9-Asn10. The first and last modules ...