Biofilm formation, antimicrobial assay, and toxin-genotypes of Clostridium perfringens type C isolates cultured from a neonatal Yangtze finless porpoiseAntibiotic resistanceBiofilm formationClostridium perfringens type CNeophocaena asiaeorientalis asiaeorientalisYangtze finless porpoise...
Then, the plates were incubated at 30 °C for 36 h, and the black colonies were selected for biofilm formation assay. To identify the location of transposon insertion, the chromosomal DNA was extracted and digested with BamHI or SpeI. The resulting fragments were self-ligated and ...
Veterinary Research 2014, 45:104 http://www.veterinaryresearch.org/content/45/1/104 VETERINARY RESEARCH RESEARCH Open Access Biofilm formation by virulent and non-virulent strains of Haemophilus parasuis Bernardo Bello-Ortí1†, Vincent Deslandes2†, Yannick DN Tremblay2, Josée Labrie2, Kate J...
Briefly, the CV assay was performed in a 24-well tissue culture plate. After biofilm formation, the medium above the surface of the biofilm was removed and 1 ml of 1% CV (HiMedia, catalogue number S012) was added to the biofilm and incubated for 10 min. The CV was removed and ...
Using crystal violet binding assay, we examined the potential for biofilm formation in 194, 76 and 107 isolates from urine of patients with uncomplicated a... Sojun Kanamaru and Hisao Kurazono and Akito Terai and Koichi Monden and Hiromi Kumon and Yoshimitsu Mizunoe and Osamu Ogawa and Shingo ...
Biofilm formation was detected using the XTT assay. The isolates were classified according to the literature as strong, moderate, or weak biofilm producers. The results indicated that 85.0% (34 of 40) of the isolates were strong biofilm producers, which included both S aureus (100.0% of the ...
Biofilm formation in 92 unrelated strains of Acinetobacter baumannii isolated in a multicentre cohort study was investigated using a microtitre plate assay. Fifty‐six (63%) isolates formed biofilm. These isolates were less frequently resistant to imipenem or ciprofloxacin than were non‐biofilm‐...
Biofilm formation assay A static biofilm formation assay was performed in 96-well polystyrene microtiter plates as previously described with slight modifications [17]. In brief, cells from overnight cultures were standardized to an optical density at 600 nm (OD600) of 0.05. Two hundred microliters...
An assay of biofilm formation in vitro was performed in glass tubes. It was found that the biofilm of the P. polymyxa HY96-2 wild-type strain was visible to the naked eye on the 2nd day after inoculation, and the biomass of the biofilm reached its maximum on the 5th day. The biofilm...
Coaggregation between F.nucleatum and Prevotella species was determined by visual assay. Effect of co-culture of the species on biofilm formation was assessed by crystal violet staining. Effect of soluble factor on biofilm formation was also examined using culture supernatant and two-compartment co-...