beta Actin Loading Control Antibody (MA5-15739-D680) in WB Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), C2C12 (Lane 4), MDA-MB-231 (Lane 5), PC-12 (Lane 6), RSC96 (Lane 7), tissue extracts...
Anti-beta-Actin (Loading Control)抗体,β-肌动蛋白/β-Actin 抗体(内参抗体) 来源:Mouse 克隆类型:Monoclonal浓度:1mg/ml亚型:IgG应用: WB=1:1000 ELISA=1:1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 IP=1:100 Flow-Cyt=1:100-200反应: Human 免疫原:KLH conjugated synthetic peptide ...
Beta-actin – A special control that matters. In Western blotting, beta-actin is commonly chosen as a loading control due to its broad and consistent expression across all eukaryotic cell types
Why is beta-actin commonly used as a control in Western blots? Loading controls are essential for proper interpretation of Western blots. They can be used to normalize the levels of protein detected by confirming that protein loading is the same across the gel. In Western blotting, beta-actin...
beta-Actin小鼠单抗说明书 We focus on precise protein quantification www.dia-an.cn 1043744051@qq.com 35kDa 25kDa 45kDa 65kDa 15kDa 10kDa 100kDa 130kDa 75kDa 170kDa Western Blotting of Hela lysates with anti-Beta Actin monoclonal antibody at dilution of 1:1000 ...
beta-Actin are useful as loading controls forWestern Blotting. However it should be noted that levels of beta-Actin may not be stable incertain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore itshould not be used as loading control for these tissues....
Vigelso A, Dybboe R, Hansen CN, Dela F, Helge JW, Guada- lupe Grau A (2015) GAPDH and b-actin protein decreases with aging, making Stain-Free technology a superior loading control in Western blotting of human skeletal muscle. J Appl Physiol 118:386-394...
beta Actin Antibody (MA5-15739-HRP) in WB Western blot analysis of Beta-Actin was performed by loading 30 µg of various cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Th...
Lane 1: Western blot - Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB8226) at 1/1000 dilution Lane 2: Western blot - Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB8226) at 1/10000 dilution Lanes 3 - 4: Western blot - Anti-beta Actin antibody [mAbc...
It has been widely used as the internal control in RT-PCR and Western Blotting. The isotype of this antibody is IgG2b. What is the molecular weight of beta-actin? 42 kDa What is the function of beta-actin? Actins are a key part of the cytoskeleton and are involved in cell motility...