自噬(autophagy)是一种进化保守的溶酶体依赖性分解代谢途径,是细胞维持自稳态的重要机制之一,并参与多种疾病的发生.Beclin-1作为自噬体成核的关键分子之一,是1个调节自噬的关键靶点.Beclin-1有1个BH3结构域,Bcl-2、Bcl-XL等可以通过这个BH3结构域与Beclin-1结合而影响其活性.抗凋亡Bcl-2家族蛋白和Beclin-1的...
( autophagy) , 摘要 自噬 是一种进化保守的溶酶体依赖性分解代谢途径 是 细胞维持 自稳态的重要 ,. Beclin-1 , 1 机制之一 并参与多种疾病的发生 作为 自噬体成核 的关键分子之一 是 个调节 自噬的 关键靶 点. Beclin-1 有 1 个 BH3 结构域 ,Bcl-2 、Bcl-XL 等可以通过这个 BH3 结构域与...
The growth-promoting activity of this Bcl-2 mutant is strongly correlated with its suppression of Beclin1-dependent autophagy, leading to sustained p62 expression and increased DNA damage in xenograft tumors, which may directly contribute to tumorigenesis. Thus, the anti-autophagic property of Bcl-2...
Ambral是新发现的一种蛋白,对依赖Beclin-1的自噬有正面调节作用,游离的Ambral与Beclin-1结合,能促进细胞自噬囊泡膜的集聚,诱导自噬的形成[12]。Bcl-2含有与Beclin-1相同的结构域BH3,Bcl-2可通过此结构域与Beclin-1结合,当Bcl-2与Beclin-1形成复合体后,可抑制自噬;当Bax竞争性结合Bcl-2后游离Beclin-1,从而...
产品介绍Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and norma...
Bcl-2 Antiapoptotic Proteins Inhibit Beclin 1-Dependent Autophagy, ArtículoApoptosis and autophagy are both tightly regulated biological processes that play a central role in tissue homeostasis, development, and disease. The anti-apoptotic protein, Bcl-2, interacts with the evolutionarily conserved ...
采用透射电镜观察细胞线粒体形态和自噬情况(仅设对照组、GLA5μg/mL组);采用JC-1染色和荧光倒置显微镜观察并检测细胞线粒体膜电位;采用Westernblot法检测细胞中Bcl-2、Bax、Beclin1、活性半胱氨酸天冬氨酸蛋白酶3(cleavedcaspase-3)蛋白的表达;采用免疫共沉淀法检测细胞中Bcl-2与Beclin1的结合与解离(仅设GLA5μ...
Immunolocalization of beclin 1, a bcl-2-binding, autophagy-related protein, in the human ovary: possible relation to life span of corpus luteum[J] . María Gaytán,Concepción Morales,José E. Sánchez-Criado,Francisco Gaytán.Cell and Tissue Research . 2008 (2)...
Moreover, mice deficient in the anti-ageing protein klotho3 have increased beclin 1 and BCL2 interaction and decreased autophagy. These phenotypes, along with premature lethality and infertility, are rescued by the beclin 1(F121A) mutation. Together, our data demonstrate that disruption of the ...
高维娟 △(河北中医学院/河北省中医药防治心脑血管病基础研究重点实验室,河北石家庄 050091)【关键词】 细胞凋亡;细胞自噬;Bcl-2;Beclin1;Bcl-2/Beclin1复合物【中图分类号】 R34 【文献标识码】 A 【文章编号】 1004-6879 (2019)04-0335-06细胞凋亡(apoptosis)和细胞自噬(autophagy)是细胞自我销毁的两种主要...