Whole genome sequencing of Bacillus velezensis LS69 will provide a basis for elucidation of its biocontrol mechanisms and facilitate its applications in the future.doi:10.1016/j.jbiotec.2017.03.018Liu, GuoqiangKong, YingyingFan, YajingGeng, Ce...
Intratumoral and intracellular microbes may also regulate the metastatic cascade. Whole genome sequencing revealed a similarity of 99.9% betweenF. nucleatumisolates from primary CRC and liver metastasis, indicating likely bacterial migratory characteristics between these tumor sites49. Microbial regulation of ...
(GenBank: WP_032874795.1). In addition, based on these data, two real-time PCR methods, that can be used by enforcement laboratories, specific to this unauthorized genetically modified bacterium were developed and validated. The present study emphasizes the key role that whole-genome sequencing ...
Bacterial Whole-Genome Sequencing Revisited: Portable, Scalable, and Standardized Analysis for Typing and Detection of Virulence and Antibiotic Resistance ... Multidrug-resistant nosocomial pathogens present a major burden for hospitals. Rapid cluster identification and pathogen profiling, i.e., of ...
ability, were also analyzed. Whole genome sequencing of the tolerant isolates was performed to determine the genes responsible for conferring resistance to As. The indigenous As-tolerant bacteria analyzed in this study are the first to be reported from the Uttar Dinajpur region of West Bengal, In...
Whole genome phylogenies of the Genome Taxonomy Database (GTDB) indicate that the SAR202 group I cells we cultured represent isolates of a hitherto-uncultured order (UBA1151) within a monophyletic superorder comprised of all SAR202 (Fig.1band Supplementary Notes). We propose the provisional taxono...
Whole metagenome sequencing estimates the abundance of ARGs,virulence genes, and mobilegeneticelements such astransposons, plasmids, and bacteriophages within a sample. Virulence genes code for bacterial motility,cell adherence, and secretion. Therefore, whole metagenome sequencing determines the ability of ...
41Altmetric Metrics Abstract CRISPR interference (CRISPRi) is a powerful tool to link essential and nonessential genes to specific phenotypes and to explore their functions. Here we describe a protocol for CRISPRi screenings to assess genome-wide gene fitness in a single sequencing step (CRISPRi-...
Whole-genome sequencing and de novo assembly DNA extraction Isolates cultivated to stationary phase were centrifuged at 7,227gat 4 °C for 10 min, and the resulting pellets were resuspended in 1 ml of Tris-EDTA. For bacterial cell lysis, 50 µl of 10% SDS and 10 µl of pro...
The genomic DNA ofSchizoochytrium limacinumHS01 and MYA-1381 was respectively extracted using yeast genome extraction kit, and then whole genome sequencing was performed by Novogene using PacBio RS II and Illumina HiSeq 4000. The results showed that compared with MYA-1381, Schizoochytrium lima...