近日,澳大利亚沃尔特和伊丽莎·霍尔医学研究所Philip Hodgkin团队在Immunity上在线发表了题为Lineage tracing reveals B cell antibody class switching is stochastic, cell-autonomous, and tuneable的研究论文,利用系谱追踪、单细胞分析和数学模型,探索ASC和CSR 命运多样性的克隆起源。 研究人员首先在体外激活刺激B细胞分化...
近日,澳大利亚沃尔特和伊丽莎·霍尔医学研究所Philip Hodgkin团队在Immunity上在线发表了题为Lineage tracing reveals B cell antibody class switching is stochastic, cell-autonomous, and tuneable的研究论文,利用系谱追踪、单细胞分析和数学模型,探索ASC和...
Single-cell repertoire tracing identifies rituximab refractory B cells during myasthenia gravis relapses. JCI Insight 5, e136471 (2020). Article PubMed PubMed Central Google Scholar Krumbholz, M. et al. BAFF is produced by astrocytes and up-regulated in multiple sclerosis lesions and primary ...
Oh, J. E. et al. Intranasal priming induces local lung-resident B cell populations that secrete protective mucosal antiviral IgA.Sci. Immunol.6, eabj5129 (2021).Evidence of lung IgA+BRMcell and IgA+plasma cell requirements for protection against influenza virus infection independent of T cells....
(IGH) V genes in the response to all sub-determinants of the spike examined, with similar characteristics observed in both donors.IGHrepertoire sequencing and B cell lineage tracing at longitudinal time points reveals extensive evolution of SARS-CoV-2 spike-binding antibodies from acute infection ...
Hence, several single cell methods are being increasingly used for B cell-specific lineage tracing and to resolve phenotypes, interactomes, and plasticity at the single cell level in a high-throughput manner. These methods can be divided into those that analyze (i) transcriptomic data, such as ...
Step 4. For each CF that was selected in Step 1, we reconstructed the B-cell lineage tree using the maximum parsimony method from Alakazam [13]. To facilitate this, we used only sequences of identical length, i.e., we removed the sequences that were different from the most common seque...
[26]. While B1 cell progenitors are present in adult bone marrow, several lines of evidence indicate that their contribution to adult B1 cell pool is quite limited under physiological condition [26,27,28,29]. A recent lineage tracing experiment with the hematopoietic stem cell restricted Pdzk1...
Β-细胞的再生：复制，干细胞分化以及小分子在其中扮演的角色 糖尿病 一型糖尿病（T1D）：先天性，儿童，B细胞缺失或者异常二型糖尿病（T2D）：后天性，成人，B细胞异常 T1D治疗新方法：β-细胞代替治疗 •胚胎干细胞•成熟的胰岛细胞•组织干细胞 血统追踪研究（Lineagetracingstudies）：2004年nature文章中...
GABA added to macrophages differentiated under neutral conditions (M-0) increased cell number, cell viability and expression of folate receptor β (FRβ), which characterizes anti-inflammatory macrophages24 (Fig. 4a, b, and Extended Data Fig. 6a–c). Gene transcripts related to the cell cycle ...