近日,Broad研究所的Vijay G. Sankaran教授利用线粒体DNA多样性特性,结合单细胞测序技术(scRNA-seq)及单细胞染色质开放性测序技术(scATAC-seq),在单细胞水平上,对线粒体DNA作为内源性条形码进行谱系追踪的可应用性进行了探索。该工作以Lineage Tracing in Humans...
摘要 细胞谱系示踪(celllineagetracing)是指利用各种方式标记细胞,并对包括其后代所有细胞 的增殖、分化以及迁移等活动进行追踪观察。自20世纪以来,谱系示踪技术为研究器官发育、组织 损伤修复以及单细胞的分化命运提供了重要的手段。近些年,随着基因工程技术的飞速发展,细胞 ...
Rempfler, M., Kumar, S., Stierle, V., Paulitschke, P., Andres, B., Menze, B.H.: Cell lineage tracing in lens-free microscopy videos. In: MICCAI (2017)M. Rempfler, S. Kumar, V. Stierle, P. Paulitschke, B. Andres, and B. Menze. Cell lineage tracing in lens-free ...
记录、追踪并绘制细胞发育历史的过程被称为谱系追踪(lineage tracing)。近年来科学家通过发展谱系追踪的方法学,深入理解了干细胞驱动的器官再生与稳态,推动了体外迷你器官培养的进步。在癌症领域,谱系追踪揭示了肿瘤细胞的起源和克隆演化,提...
Lineage-tracing experiments that rely onin vivolive imaging of cells as they divide. Determinate In the context of developmental processes, when the relationship between lineage and molecular state is tightly controlled at each cell division event and is invariant between individuals. ...
Fig. 1: Fluctuating methylation status as a lineage tracing marker. a, Illustration of the three possible methylation states at a specific CpG locus within a particular cell. A cell can either be homozygously (de)methylated or heterozygously methylated at that CpG locus. It is the spontaneous ...
Fig. 1: Fluctuating methylation status as a lineage tracing marker. a, Illustration of the three possible methylation states at a specific CpG locus within a particular cell. A cell can either be homozygously (de)methylated or heterozygously methylated at that CpG locus. It is the spontaneous ...
前瞻性谱系示踪方法，例如慢病毒条形码，它涉及用独特的 DNA 条形码标记细胞。然而，这种“静态”条形码策略通常仅限于在体外和单个时间点引入多种标签。因此，它们无法在体内捕获关键的过程。 2021年6月10日，来自美国宾夕法尼亚大学的研究团队在 Cancer Cell 期刊发表了题为：Single-cell lineage tracing of ...
所以一旦Dox诱导,Cas9开始表达,特定的gRNA便能target到evolving region的barcode的,Evolving barcode edits are encoded and inherited as cells divide,进而获得细胞的谱系发育关系。 Figure 1 macsGESTALT for high-resolution lineage tracing 3.Aggressive clones are rare and transcriptionally divergent 接着他们利用这个...
多细胞生命是从单个细胞开始,通过细胞分裂和分化,最终形成组织分明、井然有序的个体。在这个过程中细胞的发育历史被称为谱系 (lineage)。记录、追踪并绘制细胞发育历史的过程被称为谱系追踪 (lineage tracing) 。近年来科学家通过发展谱系追踪的方法学,深入理解了干细胞驱动的器官再生与稳态,推动了体外迷你器官培养的...