Molecular or environmental properties of a cell that correlate with — or could be used to predict — a cell fate decision, which are obscured from a state manifold. Direct observations Lineage-tracing experiments that rely onin vivolive imaging of cells as they divide. ...
Contemporary single-cell lineage-tracing (scLT) methods use expressed, heritable DNA barcodes to combine cell lineage readout with single-cell transcriptomics. However, reliance on transcriptional profiling limits adaptation to other single-cell assays. With CellTag-multi, we present an approach that ...
Understanding, predicting, and manipulating cell fate has been a long-sought goal of developmental and regenerative biology. Recent insights obtained from single-cell genomic and integrative lineage-tracing approaches have further aided to identify molecular features predictive of cell fate. In this ...
做了算是有一些的单细胞数据分析了,越来越对lineage tracing感兴趣,发育生物学或者说整个生物学最基本最终极的研究其实我觉得就是细胞的命运决定。一个细胞在未来会变成什么细胞(Lineage tracing),在发生转变的过程中其内部状态会发生怎么的变化(single-cell omics)。通过这两个强有力的工具,对于每一个细胞我们真的...
3.1Viral Barcoding-Based Lineage Tracing 通过病毒对细胞引入 cell-specific DNA barcodes,并在很多研究体系中得到应用 (Fig 3A)。 3.2 Cre-Lox-Based Lineage Tracing 该类方法最开始是联合fluorescent proteins和Cre-Lox system,相比较于Viral Barcoding-Based Lineage Tracing,其优势在于cell labeling can be induced...
就可以做 lineage tracing。 一旦完成这个过程之后最后得到的细胞之间的关系有三种其中State 表示细胞的状态(大概在这边可以说是指表达谱),fate 就是指细胞分化的结果。第一种就是最开始的 sister cells,第二种就是可以把原来的细胞,和它 sister cell 最后分化的细胞,第三种就是 sister cells 分化之后的细胞。
1 While classic techniques for lineage tracing, e.g., via Cre-mediated reporter expression or T cell receptor or B cell receptor diversification in lymphocytes have been used for decades, recent years have seen an explosion in new approaches.2,3 These newer approaches include the use of “...
The in vitro differentiation of insulin-producing beta-like cells can model aspects of human pancreatic development. Here, we generate 95,308 single-cell transcriptomes and reconstruct a lineage tree of the entire differentiation process from human embry
Viral Barcoding-Based Lineage Tracing;2.Cre-Lox-Based Lineage Tracing method 3.CRISPR-Cas9 Genome Editing-Based Lineage Tracing。这里打卡Viral Barcoding-Based Lineage Tracing,基于病毒感染的谱系示踪方法,题目是Single-cell lineage tracing unveils a role for TCF15 in haematopoiesis,之前有看过,但是没有很...
Here we present CRISPR-UMI, a single-cell lineage-tracing methodology for pooled screening to account for cell heterogeneity. We generated complex sgRNA libraries with unique molecular identifiers (UMIs) that allowed for screening of clonally expanded, individually tagged cells. A proof-of-principle ...