单克隆B细胞、单克隆浆细胞和移植后发生PTLD的天数与EBV相关PTLD具有相关性。 移植后淋巴细胞增殖性疾病(post-transplantation lymphoproliferative disease,PTLD)是异基因造血干细胞移植(allogeneic hematopoietic stem cell transplantation,allo-...
利用单克隆抗体和流式细胞仪技术(flow cytometry)可以区分出这些不同的细胞表面分子,从而告诉我们这是何种细胞。这些表面抗原分子被称为分化簇(cluster of differentiation,CD)。一般以CD加数字命名(要背死人了!)。在骨髓中,多能造血干细胞(HSC, CD34+)分化成为普通淋巴祖细胞(CLP, CD10+),接着发育出B cell ...
The relevance of a positive T cell flow cytometry crossmatch (FCXM) in renal transplantation is well documented, especially among regrafts and highly sensitized patients. By contrast, the relevance of a positive B cell cytotoxic or FCXM in clinical transplantation is controversial. To determine ...
未刺激组不加 LPS,其余同。对于同时检测 B10 和 B10 细胞祖细胞(B10Pro cell)的实验,需培养 48h,使 B10Pro 细胞发育为成熟的 B 细胞,在最后 5h 再加入上述刺激剂和阻断剂[14]。四、流式检测(四色法) 1. 细胞计数,取未刺激(A 管)和刺激(B 管)的细胞(2×105-2×108/管),加入适量(具体参照说明书)...
参考文献:Seegmiller AC, Hsi ED, Craig FE. The current role of clinical flow cytometry in the evaluation of mature B-cell neoplasms. Cytometry B Clin Cytom. 2018 Dec 13. doi: 10.1002/cyto.b.21756. 本文来自流式中文网(flowcyto.cn)欢迎转发到朋友圈,但谢绝复制粘贴转载如有需要请联系mail@flow...
including viruses. In this chapter we present a simple, flow cytometry based, method that determines the average avidity of GC B cells for the influenza A virus hemagglutinin glycoprotein, the target antigen of traditional influenza vaccines. Flow cytometry using fluorescent hemagglutinin and B cell ...
弥漫大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)是最常见的非霍奇金淋巴瘤[1],疾病异质性强[2],侵袭性高[3],弥漫型侵犯骨髓[4],患者在使用R-CHOP方案[5, 6, 7]及个体化精准治疗后[8, 9],仍有部分患者治疗后无效...
B cell immunophenotyping with flow cytometry Cytokine and chemokine profiling References B cell development and markers B cell development in specific organs and niche environments is coordinated by expression of transcription factors [2, 3, 4, 5, 6, 7]. Each cell fate depends on...
Targeting Cell-Bound MUC1 on Myelomonocytic and Monocytic Leukemias and Leukemic Stem Cells: Therapeutic Implications One of them, a partially humanized murine mAb termed DMB-5F3 was used to examine the expression of MUC1 on AML cells by flow cytometry. A series of twenty-two AML samples (bloo...
Villablanca研究组在Immunity上发表题为B cell expansion hinders the stroma-epithelium regenerative cross talk during mucosal healing的文章,发现B细胞通过阻碍上皮细胞和基质细胞之间的相互作用,在肠道损伤修复过程中起到了一定的负面影响。 肠道上皮细胞(Intestinal epithelial cells,简称IECs)持续进行自我更新,这一...