The practical aspects of flow cytometry and sorting are emphasized in this book which introduces the beginner to the technology and provides tips and tricks for the advanced user. The clear structure makes it easy to address specific problems fast. The chapters cover the modern applications of thes...
Flow Cytometry: Principles and Applications. Totowa, NJ: Humana Press, 2007, 257 -276.Davies, D., Cell sorting by flow cytometry. 2007: Springer. 257-276.Davies, D. (2007). Cell Sorting by Flow Cytometry. In Flow Cytometry. New York: Humana Press....
流式细胞仪的cell sorting是很多细胞和分子生物实验的基础,比如单细胞测序、细胞周期等。 Flow cytometry and cell sorting are two distinct yet complementary techniques. Both rely on antibodies to detect specific cells within a heterogeneous population, but while flow cytometry measures the proportion of each...
Flow cytometry-based cell sorting has its pros and cons. The advantages are clear: Researchers can separate cells based on a large number of variables that go beyond surface markers, such as size and metabolic status. This technique also has a high throughput, with the ability to analyze—lite...
Flow cytometry is a single-cell technique, so having a good single-cell suspension is critical for good results when sorting. Additionally, since the nozzle is smaller than it is on a typical analyzer, clumps will lead to clogs, which slow down the sort and cause unnecessary grief. ...
cell sortingFACSflow cellssheathless flow cytometrysingle cell diagnosticsIn this review, a brief history of this unrivaled technology, flow cytometry, is provided, highlighting its past and present advances, with particular focus on "flow cell" technologies. Flow cytometry has truly revolutionized high...
Traditional methods of cell sorting through flow cytometry operate through the identification and quantification of certain biomarkers (for example, proteins) on or within a cell. The method described in the study, called BD CellView Image Technology, can capture multiple images of individual cells f...
Sorting is a sample-prep starting-point While people often refer to flow cytometry and FACS (fluorescence-activated cell sorting) interchangeably, FACS specifically refers to the technology associated with cell separation. “FACS is, in principle, the same as flow cytometry, and follows the same im...
Table 1. Popular applications of flow cytometry and/or cell sorting. The Hardware Cell Biologists need to understand their projected needs, as well as budget, when determining what instrument they will need. Table 2 will show the choices in the marketplace, but since these instruments are consta...
Garner DL, Evans KM, Seidel GE (2013) Sex-sorting sperm using flow cytometry/cell sorting. Methods Mol Biol 927:279-295.Garner, D.L.; Evans, K.M. and Seidel, G.E. (2013).Sex-sorting sperm using flow cytometry/cell sorting. Methods Mol. Biol.927:279-95....