Flow cytometry and laser-activated cell sorting are unique techniques that permit the identification, analysis, and purification of individual cells based on the expression of specific markers. Flow cytometers
Cell sorting +++ - Flow cytometry: physical separation and collection of user-defined cell populations with specialized sorterMicroscopy: not possible Kinetic/time-lapse data + ++ Flow cytometry: possible but challen...
flow cytometrypulse processorselectronic detectorsfluidic systemsamplifiersSummary This chapter contains sections titled: Introduction Components of a Flow Cytometer How the Flow Cytometer Works Fluorescence-Activated Cell Sortingdoi:10.1002/9780470631119.ch1H. Krishnamurthy...
The basics of staining for cell surface proteins. In: Diamond R.; DeMaggio S. (eds) In living color: protocols in flow cytometry and cell sorting. Springer, Berlin, pp 142–164; 2000.Givan, A.L., 2000. The basics of staining for cell surface proteins. In: Diamond, R., DeMaggio,...
The basics of staining for cell surface proteins. In: Diamond R.; DeMaggio S. (eds) In living color: protocols in flow cytometry and cell sorting. Springer, Berlin, pp 142–164; 2000.Givan, A.L., 2000. The basics of staining for cell surface proteins. In: Diamond, R., DeMaggio,...
In flow cytometry facilities, biological safety assessment encompasses known hazards based on the biological sample and associated risk group, as well as potential or unknown hazards, such as aerosol generation and instrument 'failure modes'. Cell sorting procedures undergo clearly defined biological ...