Flow cytometry was used for cell sorting and detection of cell apoptosis. The invasive ability of HCT-8 cells was also observed. Results: The proliferation, migration and invasive capacity of the HCT-8 cells transfected with siRNA paxillin plasmids were inhibited. Overexpression of wild-type ...
We report on a reduced cost, portable and compact prototype design of lensless holographic microscope with an illumination/detection scheme based on wavelength multiplexing, working with single hologram acquisition and using a fast convergence algorithm
Droplet-based single-cell RNA-seq has emerged as a powerful technique for massively parallel cellular profiling. While this approach offers the exciting promise to deconvolute cellular heterogeneity in diseased tissues, the lack of cost-effective and use
The expression of paxillin mRNA and protein was analyzed, and cell proliferation and adhesion were measured. Flow cytometry was used for cell sorting and detection of cell apoptosis. The invasive ability of HCT-8 cells was also observed. Results: The proliferation, migration and invasive capacity ...
Studies on human basophils are limited by the high cost on the isolation of human basophils by magnetic-activated cell sorting (MACS) for negative depletion of non-basophils, followed by CD123-based positive selection of basophils. Moreover, such CD123-based purification of basophils may be ...
Our previously developed Red-to-Green Gene Swapping Method (RGGSM), a cytometry-based Cell-Sorting protocol, revolves around the transient expression of a green fluorescent cytoplasmic marker, to subsequently obtain purified untagged rMVA upon loss of that marker by site-specific recombination. The ...
The expression of paxillin mRNA and protein was analyzed, and cell proliferation and adhesion were measured. Flow cytometry was used for cell sorting and detection of cell apoptosis. The invasive ability of HCT-8 cells was also observed. Results: The proliferation, migration and invasive capacity ...
Briefly, fluorescence-activated cell sorting (FACS) was performed to separate the lymphocytes into CD4+ and CD8+ T-cell subsets prior to sequencing, using the amplicon-rescued multiplex (ARM)-PCR (iRepertoire Inc., Huntsville, AL, USA). We also analyzed data from a study of peripheral blood ...
Although human iPS-RPE cells failed to suppress the T cells (rather than T-cell stimulation) when we prepared fewer RPE cells for the assay (i.e., T cell:RPE ratio = 200:1, 100:1 or 50:1), iPS-RPE cells significantly suppressed the IFN-γ production of T cells from the PBMC of...