流式细胞仪的cell sorting是很多细胞和分子生物实验的基础,比如单细胞测序、细胞周期等。 Flow cytometry and cell sorting are two distinct yet complementary techniques. Both rely on antibodies to detect specific cells within a heterogeneous population, but while flow cytometry measures the proportion of each...
Multicolor Flow Cytometry Tools BD Horizon Brilliant™ Polymer Dyes 多色流式细胞术提供了各种目标细胞亚群的快速、多参数分析。其应用包括免疫学、免疫肿瘤学、病毒学、免疫监测等。BD Biosciences开发了跨越每条激光线的创新染料,以提供多色实验方案设计灵活性和全面的偶联抗体组合,帮助深度表征表面、细胞内或分泌标...
cell sortingFACSflow cellssheathless flow cytometrysingle cell diagnosticsIn this review, a brief history of this unrivaled technology, flow cytometry, is provided, highlighting its past and present advances, with particular focus on "flow cell" technologies. Flow cytometry has truly revolutionized high...
Flow cytometry-based cell sorting has its pros and cons. The advantages are clear: Researchers can separate cells based on a large number of variables that go beyond surface markers, such as size and metabolic status. This technique also has a high throughput, with the ability to analyze—lite...
For more than 45 years, we at BD Biosciences have dedicated ourselves to advancing science and improving lives through flow cytometry. Our flow cytometry expertise, world class support and cutting-edge technology enable the routine or complex fluorescence activated cell sorting (FACS) analysis needed ...
Some methods can be used in flow cytometry cell sorting: Mechanical sorting Some flow cytometers cell sorter use a mechanical device called the catcher tube to sort the cells of interest. The catcher tube is located in the upper portion of the flow cell and moves into the stream to collect...
“However, after FACS, the cells of interest are retained for further study rather than being discarded.” Nick Trotta, director, product applications and market development at Cell Microsystems, adds that flow cytometry can be seen as an analytical endpoint, whereas sorting is a starting point ...
Flow cytometry is a single-cell technique, so having a good single-cell suspension is critical for good results when sorting. Additionally, since the nozzle is smaller than it is on a typical analyzer, clumps will lead to clogs, which slow down the sort and cause unnecessary grief. ...
Traditional methods of cell sorting through flow cytometry operate through the identification and quantification of certain biomarkers (for example, proteins) on or within a cell. The method described in the study, called BD CellView Image Technology, can capture multiple images of individual cells f...
Flow cytometry was always a bit blind because you can't see exactly what is going on. Is your dye on the surface, or is it intracellular? Do you have nuclear expression? Have your particles internalized in the cell, or are they sticking to the outside? It’s not possible to answer qu...