The practical aspects of flow cytometry and sorting are emphasized in this book which introduces the beginner to the technology and provides tips and tricks for the advanced user. The clear structure makes it easy to address specific problems fast. The chapters cover the modern applications of thes...
Find a wealth of information about how flow cytometry works, FACS instruments and reagents, resources and tools for panel design to help you with multicolor flow.
cell sortingFACSflow cellssheathless flow cytometrysingle cell diagnosticsIn this review, a brief history of this unrivaled technology, flow cytometry, is provided, highlighting its past and present advances, with particular focus on "flow cell" technologies. Flow cytometry has truly revolutionized high...
流式细胞仪的cell sorting是很多细胞和分子生物实验的基础,比如单细胞测序、细胞周期等。 Flow cytometry and cell sorting are two distinct yet complementary techniques. Both rely on antibodies to detect specific cells within a heterogeneous population, but while flow cytometry measures the proportion of each...
Flow cytometry is a single-cell technique, so having a good single-cell suspension is critical for good results when sorting. Additionally, since the nozzle is smaller than it is on a typical analyzer, clumps will lead to clogs, which slow down the sort and cause unnecessary grief. ...
Flow cytometry-based cell sorting has its pros and cons. The advantages are clear: Researchers can separate cells based on a large number of variables that go beyond surface markers, such as size and metabolic status. This technique also has a high throughput, with the ability to analyze—lite...
“However, after FACS, the cells of interest are retained for further study rather than being discarded.” Nick Trotta, director, product applications and market development at Cell Microsystems, adds that flow cytometry can be seen as an analytical endpoint, whereas sorting is a starting point ...
Traditional methods of cell sorting through flow cytometry operate through the identification and quantification of certain biomarkers (for example, proteins) on or within a cell. The method described in the study, called BD CellView Image Technology, can capture multiple images of individual cells f...
As with all of our flow cytometry standard microspheres, the Cell Sorting Set-Up Beads are stained internally rather than on the surface. The dyes are therefore insulated from environmental interactions that could cause variable fluorescence o...
Garner DL, Evans KM, Seidel GE (2013) Sex-sorting sperm using flow cytometry/cell sorting. Methods Mol Biol 927:279-295.Garner, D.L.; Evans, K.M. and Seidel, G.E. (2013).Sex-sorting sperm using flow cytometry/cell sorting. Methods Mol. Biol.927:279-95....