CLINICAL FLOW CYTOMETRY CROSSMATCH: DETECTION OF DONOR-T CELL, -B CELL, AND -ENDOTHELIAL PROGENITOR CELL SPECIFIC (EPC) IGG AND IGM ANTIBODIES IN TRANSPLANT RECIPIENT SERUM.The AbSorber flow cytometry crossmatch (FXM) XM-ONE 3:1 (XM3) test measures binding of IgG and IgM antibodies (ab) in...
在感染,接种疫苗或是输血,妊娠,移植后,通过 B 细胞受体(B cell receptor,BCR)对外来抗原的 识别,成熟的初始 B 细胞会被激活,并分化为记忆 性 B 细胞和浆细胞,使机体获得免疫记忆功能[16]. 维持机体免疫记忆功能的细胞主要是记忆性 B 细 胞和长寿浆细胞,且这种免疫记忆的维持是不依赖 于抗原的[17].长寿浆...
Class-switch recombination (CSR) is an integral part of B cell maturation. Here we present sciCSR (pronounced ‘scissor’, single-cell inference of class-switch recombination), a computational pipeline that analyzes CSR events and dynamics of B cells from single-cell RNA sequencing (scRNA-seq) ...
This chapter discusses the use of flow cytometry within the environmental microbiology laboratory. It focuses on flow cytometric methods for the detection ... G Vesey,J Narai,N Ashbolt,... - 《Methods in Cell Biology》 被引量: 135发表: 1994年 Flow Cytometry Protocols With rapid improvements in...
We collected diagnostic flow cytometry data, including the proportion of T cells, abnormal B cells, normal B cells, plasma cells, NK cells, monocytes, and granulocytes in fresh biopsy tissues at clinical presentation, and analyzed the correlations with patient survival and between different cell pop...
Fig. 3: B cell immunoprofiling of globalGpr55-deficient mice after 4 weeks of WD. a, Splenic B cell subsets quantified by flow cytometry (n = 15–16; *P < 0.05).b,c, Representative gating strategy and relative proportion of B1 cell subsets.d,e, Representative gating strategy...
After flow cytometric analysis, heterogeneous cell populations were reproducibly resolved into HIV-1-positive and -negative distributions. Fluorescence microscopy ... B Patterson,M Till,P Otto,... - 《Science》 被引量: 1866发表: 1993年 Immunofluorescent analysis of blood cells by flow cytometry His...
Distribution of Thy memory IgG+ B-cell and BM memory B-cell genes in the molecular subtypes of DLBCL designated GCB-, ABC-, and UNC-DLBCL compared with PMBL. A chi-squared test was performed for overrepresented and underrepresented Thy memory B-cell and BM memory B-cell genes in PMBL comp...
麦冬皂苷B抑制人食管鳞状细胞癌细胞系EC9706增殖和迁移
e, f PBMCs of 43 AD patients and 41 normal subjects were harvested and subjected to flow cytometry with a panel of T cell, B cell and NK cell marker monoclonal antibodies. g The positive cell numbers of T cells, B cells and NK cells (mean ± SEM). h–j Correlation analysis of...