However, it remains challenging to monitor endogenous WNT/CTNNB1 signaling dynamics in vivo . Here we report the generation and characterization of a new knock-in mouse strain that doubles as a fluorescent reporter and lineage tracing driver for WNT/CTNNB1 responsive cells. We introduced a multi...
Axi1; Axil; Conductin NCBI ID 12006 MGI ID 1270862 Ensembl ID ENSMUSG00000000142 Pubmed Axin2 人类同源基因 AXIN2品系描述 The exon 1-3 of Axin2 gene was deleted to generate Axin2 knockout mouse. *使用本品系发表的文献需注明: Axin2-KO(1) mice (Cat. NO. NM-KO-240561) were purchased...
To investigate the role of Wnt-beta-catenin signaling in bone remodeling, we analyzed the bone phenotype of female Axin2-lacZ knockout (KO) mice. We found that trabecular bone mass was significantly increased in 6- and 12-month-old Axin2 KO mice and that bone formation rates were also sig...
These two AXIN proteins are considered functionally equivalent, as an Axin2 cDNA rescues the Axin1-null lethality in the mouse [30]. However, while the Axin1 knockout mouse is lethal in embryogenesis at e9.5 [31,32], mice carrying homozygous null mutations in Axin2 are viable and fertile...
碧云天同时提供基于CRISPR/Cas9技术的AXIN2基因敲除的质粒(L24585 pLenti-AXIN2-sgRNA)、慢病毒(L24586 AXIN2 Knockout Lentivirus)、HEK293T细胞(L24587 AXIN2 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L24588 AXIN2 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L24589 AXIN2...
AXIN2 Knockout Lentivirus(AXIN2基因敲除慢病毒)是一种感染动物细胞后可以同时表达Cas9、目的基因sgRNA和puromycin抗性基因的慢病毒。本产品用于在动物细胞中基于CRISPR/Cas9技术敲除目的基因,并且本慢病毒中sgRNA的有效性已经通过T7EI法的验证。 本慢病毒基因序列的关键图谱信息请参考图1。本慢病毒可用于感染细胞或组织...
To rule out further any such contribution, we physically scratched off the endometrial lining in two different mouse models in which Pax8 promoter drives either lacZ (Figure 2J) or the EYFP reporter (Figure S4A) expression and traced the origin of the regenerated epithelium. Histologically, the ...
knockout (cKO) mouse model, RAW264.7 cell line, and human subepicardial tissues from patients with coronary artery bypass graft (CABG).Material or subjects Axin2 cKO mice showed decreased cardiac function, reduced edema, increased lymphangiogenesis, and improved repair in MI Few studies border ...
a Immunofluorescence staining of endogenous β-catenin (green) in SW480 and SW480 AXIN2 knockout cells co-transfected with P182–195 or its QV-PS mutated analog together with mScarlet-tubulin (red) to visualize transfected cells. Scale bar: 20 µm. b, c Quantification of nuclear β-...
In addition, the expression of Snail-mediated proinflammatory cytokines, such as IL8, CCL2, and CCL5, was significantly decreased after Axin2 knock- down in OSCC cells compared to control cells. Admit- tedly, all of these cytokines are related to characteristics An et al. BMC Cancer (2020)...