LC3(light chain 3)是目前公认的自噬分子标记物(autophagy marker)。LC3最初发现时被认为是微管关联蛋白1A和1B(microtubul… www.neobioscience.com|基于3个网页 2. 自噬分子标志物 LC3(light chain 3)是目前公认的自噬分子标志物(autophagy marker)。LC3最初发现时被认为是微管关联蛋白1A和1B(microtubul… ...
Since harmol increases the nuclear translocation of TFEB, we analyzed the effect of harmol on autophagy marker LC3B (microtubule-associated protein 1 light chain 3 β) to verify the effect of harmol on autophagy flux and lysosomal biogenesis. Harmol treatment significantly enhances LC3B-II/LC3B-...
Annexin V binds to phosphatidylserine, a marker of apoptosis when it is on the outer leaflet of the plasma membrane23. Dcp-1 is a commonly used marker for cells undergoing apoptosis in Drosophila24. While we could visualize the previously described age-induced increase of apoptotic cells, no ...
與知名的自噬作用(Autophagy)標記(Marker)「LC3」具結果相關性 不需要進行轉染(Transfection),只要加入DAPGreen染劑,就可以偵測自噬小體(Autophagosome) Assay Data 使用共軛焦顯微鏡觀察螢光 (Observation on Confocal Fluorescence Microscopy) 使用流式細胞儀分析 (Analysis by Flow Cytometry) ...
which is responsible for many neurodegenerative diseases. The induction of autophagy flux by [(EuIII(OH)3)-NPs] has been observed in different cell lines such as Neuro 2a, PC12 and HeLa cells, and it was due to the expression of characteristic autophagy marker LC3-II and degradation of sel...
colocalization ofαSyn and the autophagy marker LC3 was reduced forαSyn-A30P compared with the otherαSyn variants. The number of mitochondria colocalizing with LC3 as a marker for mitophagy did not differ among the groups. In the rat optic nerve, bothαSyn-WT and -A30P accelerated kinetic...
In this chapter, we introduce several methods that rely on the analysis of LC3, a versatile marker protein of autophagic structures in mammalian cultured cells. The appearance of LC3‐positive puncta is indicative of the induction of autophagy, and it is observed either by immunofluorescence or by...
To further demonstrate the Dcp-1-regulated autophagic gene induction, we used UAS-Atg8b-GFP as an autophagy marker in the Dcp-1 GF animals. Similar to Atg5, Atg8b-GFP was induced in these animals (Figure 5J), even though Atg8b-GFP over-expression did not suppress the rough eye ...
Fig. 5. Changes of autophagy marker protein and lysosome-related gene expression levels under TFEB gene regulation during Cd or Qu treatment. Cells were transfected with control siRNA (siCt), TFEB siRNA (siTFEB), empty GV492 or plasmid GV492 carrying TFEB gene, then TFEB knockdown (A) and ...
This tremendous progress continues to depend largely on powerful research methods, specifically, various autophagy marker Atg8–PE protein-based methods for studying membrane dynamics and monitoring autophagic activity. Recently, several biochemical approaches have been successfully developed to produce the ...