Autophagy marker levels were assessed by immunoblotting. Differentially expressed autophagy-related genes between healthy and T2DM groups were identified based on a previously published RNA-sequencing data-set, which we verified by RT-qPCR. siRNA was used to assess the function of differentially ...
as well as LC3I and LC3II levels (in which an increased LC3II-to-LC3I ratio is considered a marker of autophagy activation) were not significantly different between genotypes (Supplementary
Fig. 5. Changes of autophagy marker protein and lysosome-related gene expression levels under TFEB gene regulation during Cd or Qu treatment. Cells were transfected with control siRNA (siCt), TFEB siRNA (siTFEB), empty GV492 or plasmid GV492 carrying TFEB gene, then TFEB knockdown (A) and ...
This new population (M1), when cell sorted and analyzed by western blotting, demonstrated an increased degradation of autophagy marker LC3B II [7] that accumulated upon bafilomycin A treatment, indicating that this population was undergoing more autophagy (Figures S1A and S1B). The variation in ...
Six hours after administration, echocardiography was performed to assess the success of the model, with LVFS < 50% as a marker of successful SIMD mouse modeling [13, 39]. Grouping: The mice were grouped randomly as follows (N = 12): (1) control group: injected intraperitoneally ...
Gastric juice long noncoding RNA used as a tumor marker for screening gastric cancer. Cancer. 2014;120(21):3320–8. Article CAS PubMed Google Scholar Yuan J, Zhang F, Niu R. Multiple regulation pathways and pivotal biological functions of STAT3 in cancer. Sci Rep. 2015;5:17663. ...
RT-qPCR results of the antioxidant genes in jejunal mucosa showed that compared to G1, the thioredoxin reductase (TRX) gene expression in G2 was markedly down-regulated, while NAD(P)H: quinone oxidoreductase 1 (NQO-1) transcription was up-regulated. Moreover, gene expressions of SOD, ...
Melatonin, a naturally biosynthesized molecule secreted by the pineal gland, exhibits antitumor activities against several different types of cancer. The mechanisms of action of melatonin against tumor progression involve cellular apoptosis, antimetastat
(D) Dot plot showing the expression of marker genes in the 16 cell clusters; (E) Heatmap displaying the expression of marker genes in the 16 cell clusters; (F) Expression of intestinal neuron marker gene Eno2 in various cells; (G) Expression of intestinal neuron marker gene Tubb3 in ...
(Fig.1C), confirming EGFP as a transfection marker of thepEGFP-LC3BpepABLucsensor in MDA-MB-231 cells, alongside the expression of the luciferase fusion protein. To explore the broader applicability of thepEGFP-LC3BpepABLucsensor, we also transfected it into MDA-MB-468 and MDA-MB-468 TNBC...