SGC-7901 gastric cancer cell line was treated with 5-fluorouracil (5-FU) or/and autophagy inhibitor bafilomycin A1. Cell viability and growth were evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) and clonogenic assay. Apoptosis was evaluated by flow cytometry....
Exposure to 3-MA, the autophagosome formation inhibitor, did not eliminate CUR5g-induced SQSTM1 and LC3B-II accumulation (Fig. 2A). Treatment of A549 cells with CUR5g and the late-stage autophagy inhibitor CQ showed no effect on further increasing LC3B-II relative to simply CQ (Fig. 2B)....
中文名称:BAFILOMYCIN A1 自噬抑制剂, 质子泵抑制剂 中文同义词:BAFILOMYCIN A1 自噬抑制剂, 质子泵抑制剂 英文名称:BAFILOMYCIN A1 autophagy inhibitor, proton pump inhibitor 英文同义词: CAS号: 分子式: 分子量:0 EINECS号: 信息错误报告 您的Email: ...
U2OS cells expressing a Tet-regulated construct were either mock treated (control) or treated with tetracycline for the indicated times to induce the expression of constitutively active MKK6.aCells were treated with the autophagy inhibitor bafilomycin A1 (BAF) for 4 h at the indicated times afte...
Fig. 3. Concurrent treatment with enzalutamide and an autophagy inhibitor triggered apoptosis in J82 and T24 cells. (A) J82 and T24 cell lines were treated with ENZ and BAF for 48 h and divided into four groups (control (Ctrl), 20 μM ENZ, 10 nM BAF, and ENZ + BAF)....
For FOXO1 inhibition, the cells were treated for 6 hours with the specific inhibitor AS1842856 (HY-100596; MedChemExpress) at a final concentration of 0.25 μmol/L. For autophagy inhibition, Hepa 1–6 cells were treated for 12 hours with 0.1 mmol/L OA and 10 nmol/L bafilomycin A1 (HY...
inhibitors leupeptin andbafilomycin A1 on autophagic activity. Torin 1 and torin 2 inhibit mTOR complex1 and activate autophagy at an early step. Leupeptin inhibits lysosomal proteasesand inhibits autophagy at a late step. Bafilomycin A1 is an inhibitor of theV-ATPase and inhibits lysosomal ...
Oligomycin, inhibitor of the F0 part of H+-ATP-synthase, suppresses the TNF-induced apoptosis. The release of cytochrome c from the intermembrane space of mitochondria into the cytosol is one of the critical events in apoptotic cell death. In the pre... Shchepina LA,Pletjushkina OY,Avetisya...
Treatments with 1 nM of the autophagy activator rapamycin (Rapa) for 24 h alone or in combination with 300 nM of the autophagy inhibitor bafilomycin A1 (Baf) were used as controls. For all cell lines, Rapa treatment significantly increased the number of AL (red puncta) by ~1.5 to ...
(Fig.3). The uptake of Ca2+into lysosomes is not yet well elucidated. Several studies support the idea that a Ca2+/H+exchanger exists due to a notable reduction in lysosomal Ca2+when lysosomal pH is disturbed by a V-ATPase inhibitor or alkalizing reagents such as NH4Cl7,81,82,83. ...