Integrative recombination of bacteriophage lambda: extent of the DNA sequence involved in attachment site function We have investigated the minimum extent of DNA sequence required for the attachment site of bacteriophage 位 to function in integrative recombination. A DN... M Mizuuchi,K Mizuuchi - 《...
电话:400-800-2947 工作QQ:1843439339 (微信同号) 邮件:Biovector@163.com 手机:18901268599 地址:北京 已注册 详细信息 咨询记录(共0条) Name质粒名称: BioVector® pGE-attB-GMR Description描述: Map图谱: Sequence序列: Promoter启动子: Synonyms别名: ...
Plasmid Name质粒名称: p2attB-FST344-HA-CPL Gene/Insert插入基因名称: Follistatin (Homo sapiens) Cat#货号: BioVector-0131460 Map图谱: Sequence序列:Contact BioVector NTCC Inc. Supplier来源:BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心 电话:400-800-2947 ...
shRNA_sequence size1000 species 7227 Drosophila melanogaster tags resistance markers : 555 tags : Unknown more info or order: Addgene product webpage company information Addgene 490 Arsenal Way, Suite 100 Watertown, MA 02472 info@addgene.org ...
Further investigation of mutations at different positions within the attB sequence shows different recombination efficiencies and binding affinities. We found four conserved regions within the attB motif that coincide with the results of recombination assays, and mutations in the attB sequence that hamper ...
We recently devised a new application of the Bxb1 integrase strategy to meet this need through an intronic attB sequence within the gene of interest. Although this attB is silent and without effect on intron splicing or protein translation and function, it allows efficient gene modification with ...
Attb cell line, transgenic cell lines derived therefrom, and methods of making the sameA genetically modified mammalian cell and genetically modified mammalian cell line comprise a recombination sequence inserted in a target locus on a chromosome of the mammalian cell genome, wherein the recombination...
A carrier carrying an exogenous functional gene is inserted with a plurality of mutually isolated attB sequences, then, together with a carrier expressing an acteriophage phiC31 intergrase, is treated with cotransfection to a host cell, so as to realize efficient integration of attB and pseudo...
Cianciotto N, Serwold-Davis T, Groman N, Ratti G, Rappuoli R: DNA sequence homology between attB-related sites of Corynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacterium glutamicum, and the attP site of gamma-corynephage. FEMS Microbiol Lett 1990, 54:299-301....
Second, a recombination reaction is inhibited by the introduction of varying amounts of a second attB with a sequence change that blocks recombination with this site. The inhibition constant reflects the dissociation constant of the intasome and altered attB in this experiment. The two methods ...