Amplification of the attB and attP sequences generated after the three types of excision from PAISt.Jose, C. HuguetTapiaDawn, R. D. BignellRosemary, Loria
We have investigated the minimum extent of DNA sequence required for the attachment site of bacteriophage 位 to function in integrative recombination. A DN... M Mizuuchi,K Mizuuchi - 《Proc Natl Acad Sci U S A》 被引量: 301发表: 1980年 Integrative recombination of bacteriophage lambda: extent...
sequence of the Streptomyces sp.IIR21 strain was of a length of 269 bp, and is most similar to the attB site core region of Streptomyces.natalensis, with similarity reaching 92%, and contain the core cross-over region 5′-TT.The integrative plasmid pSET152 ...
The invention discloses a carrier carrying an attB for efficient orientated integration of pseudo attP sites in a genome and a transgene method thereof. A carrier carrying an exogenous functional gene is inserted with a plurality of mutually isolated attB sequences, then, together with a carrier ...
DNA sequence homology between attB-related sites of Corynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacterium glutamicum, and the attP site of gamma-corynephage. FEMS Microbiol Lett. 1990 Jan 1; 54 (1-3):299–301. [ PubMed ]
在携带外源功能基因的载体中插入多个互相间隔的attB序列,和表达噬菌体φC31整合酶的载体共转染宿主细胞,可以实现attB与基因组中的假attP位点的高效整合及外源基因的表达. The insertion of foreign genes carrying multiple attB sequence vectors each interval, and the expression of the phage integrase φC31 vector...
Parallel Genomic Engineering of Two Drosophila Genes Using Orthogonal attB/attP SitesphiC31, transgenesis, Drosophila melanogaster, CRISPR, orthogonalPrecise modification of sequences in the Drosophila melanogaster genome underlies the powerful capacity to study molecular structure-function relationships in this...
Cianciotto N, Serwold-Davis T, Groman N, Ratti G, Rappuoli R (1990) DNA sequence homology between attB -related sites of Corynebacterium diphtheriae , Corynebacterium ulcerans , Corynebacterium glutamicum , and the attP site of γ-corynephage. FEMS Microbiol Lett 66:299–301...
Integration of attP-containing plasmids resulted in the incorporation of the tMx8 terminator sequence, in addition to a short sequence of Mx8 DNA downstream of trnD. The integrant was unable to process the trnD transcript at the normal 3' processing site and displayed a lower level of ...
The DNA sequence of the attB was cloned and shown to be conserved. The results of antimicrobial activity analysis indicated that the insertion of plasmid pIJ8600 seemed to affect the biosynthesis of antibiotics that could strongly inhibit the growth of E. coli and Mucor miehei (Tü284). HPLC–...