A phage genome has been isolated which contains both attB and attP, the bacterial and phage sites which are recombined in the process of formation of stable lysogens. The derivative was created by an illegitimate recombination during mixed vegetative growth of two phage strains which contained attB...
I.OCAI,IZATIONOFCOSANDattPSITESONPHAGEcpHAU3 ANDattBSITEONTHEH0STCHROM【alsoMES ZhouXiufentDengZixinTobiasKieser2DavidA. Hopwood: ActinophageHAU3hasawidehostrangeSouthernhybridizationindicatesth at mHAU3DNAcanlysogenizeStreptomyceshyoroscopicusl0—22aswllasS treptomyces lividansZX7byintegratinginto...
A phage genome has been isolated which contains both attB and attP, the bacterial and phage sites which are recombined in the process of formation of stable lysogens. The derivative was created by an illegitimate recombination during mixed vegetative growth of two phage strains which contained attB...
However, while combined modifications of non- linked genetic elements can be easily constructed with these tools and classical genetics, the independent manipulation of linked genes through the established FC31-mediated transgenesis pipeline has not been feasible due to the limitation to one attB/attP ...
The invention discloses a carrier carrying an attB for efficient orientated integration of pseudo attP sites in a genome and a transgene method thereof. A carrier carrying an exogenous functional gene is inserted with a plurality of mutually isolated attB sequences, then, together with a carrier ...
一种携带attB高效定向整合假attP位点的载体及其转基因方法 A portable and efficient directional integration false attB attP sites carrier and transgenic methodsdoi:CN102399810 BCN曾溢滔谢飞曾凡一
Amplification of the attB and attP sequences generated after the three types of excision from PAISt.Jose, C. HuguetTapiaDawn, R. D. BignellRosemary, Loria
DNA sequence homology between attB-related sites of Corynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacterium glutamicum, and the attP site of gamma-corynephage. FEMS Microbiol Lett. 1990 Jan 1; 54 (1-3):299–301. [ PubMed ]
Cianciotto N, Serwold-Davis T, Groman N, Ratti G, Rappuoli R (1990) DNA sequence homology between attB -related sites of Corynebacterium diphtheriae , Corynebacterium ulcerans , Corynebacterium glutamicum , and the attP site of γ-corynephage. FEMS Microbiol Lett 66:299–301...
attB/attP sitesGenome ReportEffective genome engineering should lead to a desired locus change with minimal adverse impact to the genome itself. However, flanking loci with site-directed recombinase recognition sites, such as those of the phage ΦC31 integrase, allows for creation of...