Nondegradative role of Atg5-Atg12/Atg16L1 autophagy protein complex in antivi- ral activity of interferon gamma. Cell Host Microbe 2012; 11: 397 - 409.Hwang, S., N.S. Maloney, M.W. Bruinsma, G. Goel, E. Duan, L. Zhang, B. Shrestha, M.S. Diamond, A...
The Atg12-Atg5-Atg16 complex interacts directly with Atg3 via Atg12, and enhances the conjugase activity of Atg3 by rearranging its catalytic center, while it is targeted to the membranes via Atg5 and Atg16, and promotes the transfer of Atg8 from Atg3 to the membranes....
Herein, we report that a molecular ATG5 “conjugation switch”, comprised of competing ATG12 and ubiquitin conjugation reactions, integrates ATG12–ATG5-ATG16L1 complex assembly with protein quality control of its otherwise highly unstable subunits. This conjugation switch is tightly regulated by ATG16...
To consolidate the Atg5-TECPR1 complex formation, we performed reciprocal affinity purification for the TECPR1 cellular complex; only the Atg12-Atg5 conjugate was present as a stoichiometric component of the TECPR1 complex (Figure 1A, right panel). Surprisingly, Atg16 or other potential associated ...
Yuan Gao1, Yanquan Wei1, Xiangtao Liu1, Ying Liao*,3, Huichen Guo*,1 and Shiqi Sun*,1 Autophagy-related protein ATG5-ATG12 is an essential complex for the autophagophore elongation in autophagy, which has been reported to be involved in foot-and-mouth disease virus (FMDV) replication...
Autophagy-related protein ATG5-ATG12 is an essential complex for the autophagophore elongation in autophagy, which has been reported to be involved in foot-and-mouth disease virus (FMDV) replication. Previous reports show that ATG5-ATG12 positively or ne
COMPLEXINSIGHTSPATHWAYAtg12 is conjugated to Atg5 through enzymatic reactions similar to ubiquitination. The Atg12-Atg5 conjugate functions as an E3-like enzyme to promote lipidation of Atg8, whereas lipidated Atg8 has essential roles in both autophagosome formation and selective cargo recognition during...
Mechanism and functions of membrane binding by the Atg5-Atg12/Atg16 complex during autophagosome formation. EMBO J. 2012;31(22):4304-17.Romanov J, Walczak M, Ibiricu I, Schuchner S, Ogris E, Kraft C, et al. Mechanism and functions of membrane binding by the Atg5-Atg12/Atg16 complex ...
Representative immunoblots of Lc3b, Beclin1, p62 and tubulin (A) and Pi3kC3 and Atg5-Atg12 complex in the TA, SOL and DIA muscles of control and LPS-treated mice.Mahroo, MofarrahiIoanna, SigalaYeting, GuoRichard, ...
membrane repairTECPR1ATG16L1 is an essential component of the Atg8-family protein conjugation machinery, providing membrane targeting for the ATG12-ATG5 conjugate. Recently, we identified an alternative E3-like complex that functions independently of ATG16L1. This complex utilizes the autophagosome-...