Different alternative combinations of exons and splice sites generated during splicing can produce multiple transcripts and different protein sequences from a single locus. This is, in part, responsible for the much greater diversity of the transcriptome and proteome than would be obtained from a ...
Next, we wondered how nobiletin downregulates AR-V7, such as by inhibiting AR-V7 protein synthesis or promoting its degradation. On one hand, real-time quantitative PCR assay was performed to detect the changes of AR-V7 mRNA in 22Rv1 cells treated with various concentrations of nobiletin, but...
转染法转染到6cm培养皿的293T细胞中,体系为:PWPI-Flag-AR或者PWPI-Flag-ARv33 2μg,PWPI-AR-v7 4μg,补水到180μL,20μl 2.5M CaCl 2 ,200μL 2XHBS。24小时候后,超声破碎细胞,用Anti-Flag抗体跟蛋白裂解液在4℃孵育2小时,然后采用protein A/G磁珠将蛋白复合物调取出来,煮沸进行WB检测。
In addition, AR-V7 protein expression was predominantly localized to the nucleus in tumor cells from patients with mCRPC. In some cases, different metastases from the same patient were heterogeneous with regard to AR-V7 expression. Finally, AR-V7–negative patients showed significantly better ...
公司成立:5年 注册资本:100万 企业类型:有限责任公司(自然人投资或控股) 主营产品:Elisa检测试剂盒、PCR检测试剂盒、抗体、重组蛋白,蛋白定制,细胞系,原代细胞,常用实验试剂,染色液等科研产品。 公司地址:松江区新桥镇卖新公路2078号8309号 进入店铺 询盘 店...
ImmunogenSynthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Androgen Receptor (AR-V7 specific) aa 600 to the Cterminus. Sequence- Gene ID367 SwissProtC0JKD3 SynonymsAIS; AR8; DHTR; HUMARA; HYSP1; KD; NR3C4; SBMA; SMAX1; TFM; AR ...
with Androgen Receptor (ARv7 Splice Variant) antiboy [HL1028] (GTX635842) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas progr...
The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-VNRVPYPSPS CVKSEMGPWM ENYSGPYGDM RLDSTRDHVL PIDYYFPPQK TCLICGDEAS GCHYGALTCG SCKVFFKRAA EGKQKYLCAS RNDCTIDKFR RKNCPSCRLR KCYEAGMTLG ARKLKKLGNL KLQEEGENSS AGSPTEDPSQ KMTVSHIEGY ECQPIFL...
Transient siRNA transfections were carried out using siRNA sequences outlined in Supplementary Table S1 using Lipofectamine RNAiMax (Thermo) according to manufacturer's protocol. The ViraPower Lentiviral Expression system (Life Sciences) was used to generate viral particles using pLenti-AR-V7 ...
Its protein expression in tumor tissue or circulating tumor cells (CTCs) has been associated with shorter overall survival (OS), disease-specific survival (DSS) and/or recurrence-free survival (RFS) [13,14,15]. However, AR-V7 protein detection is mostly focused on nuclear staining, while ...