Hence, APEX2-mediated proximity labeling of RAB neighboring proteins represents a new and efficient tool to define RAB functions.Del Olmo, TomasLauzier, AnnieNormandin, CarolineLarcher, RaphaelleLecours, MiaJean, DominiqueLessard, LouisSteinberg, FlorianBoisvert, Francois-MichelJean, Steve...
大家好,本周跟大家分享一篇发表在Angew上的文章,文章题目是“APEX2-based Proximity Labeling of Atox1 Identifies CRIP2 as a Nuclear Copper-binding Protein that Regulates Autophagy Activation”。本文通讯作者是来自北京大学药学院...
A genetically encoded peroxidase with improved sensitivity, APEX2, is reported for electron microscopy and proximity labeling at low expression levels. APEX is an engineered peroxidase that functions as an electron microscopy tag and a promiscuous labeli
近日,药学院天然药物及仿生药物国家重点实验室王晶团队在学术期刊《Angewandte Chemie International Edition》发表了题为“APEX2-based Proximity Labeling of Atox1 Identifies CRIP2 as a Nuclear Copper-binding Protein that Regulates Autophagy Activation”(利用APEX2邻近标记技术鉴定铜伴侣蛋白Atox1细胞核内铜结合蛋...
APEX is an engineered peroxidase that functions as an electron microscopy tag and a promiscuous labeling enzyme for live-cell proteomics. Because limited sensitivity precludes applications requiring low APEX expression, we used yeast-display evolution to improve its catalytic efficiency. APEX2 is far mor...
近日,药学院天然药物及仿生药物国家重点实验室王晶团队在学术期刊《Angewandte Chemie International Edition》发表了题为“APEX2-based Proximity Labeling of Atox1 Identifies CRIP2 as a Nuclear Copper-binding Protein that Regulates Autophagy Activation”(利用APEX2邻近标记技术鉴定铜伴侣蛋白Atox1细胞核内铜结合蛋...
Cre-dependent APEX2 proximity labeling in the striatum APEX2 is an engineered peroxidase that can be rapidly induced to tag proteins in minutes with biotin phenol (BP) and H2O212. To broadly target major subcellular proteomes in the mouse brain, we created and expressed three Cre-dependent APEX...
Reminiscent of their evolutionary origin, mitochondria contain their own genome (mtDNA) compacted into the mitochondrial chromosome or nucleoid (mt-nucleoid). Many mitochondrial disorders are characterized by disruption of mt-nucleoids, either by direct mutation of genes involved in mtDNA organization or...
Enzymeヽatalyzed proximity labeling (PL) with the engineered ascorbate peroxidase APEX2 is a novel approach to map organelle compartmentalization and protein networks in living cells. Current procedures developed for mammalian cells do not allow delivery of the co﹕ubstrate, biotin﹑henol, into ...
近日,药学院天然药物及仿生药物国家重点实验室王晶团队在学术期刊《Angewandte Chemie International Edition》发表了题为"APEX2-based Proximity Labeling of Atox1 Identifies CRIP2 as a Nuclear Copper-binding Protein that Regulates Autophagy Activation"(利用APEX2邻近标记技术鉴定铜伴侣蛋白Atox1细胞核内铜结合蛋白...