之前的推文中我们探讨了蛋白邻近标记技术(proximity labeling, PL)与传统Co-IP和GST pull down技术在蛋白互作研究中的优缺点及适用场景,迅速引起了大家的“围观”,详见“蛋白互作技术大比拼:蛋白邻近标记技术与Co-IP、GST pull down在研究蛋白互作中的技术优势对比”。由于Co-IP实验在细胞内研究中对抗体要求高且难以...
大家好,本周跟大家分享一篇发表在Angew上的文章,文章题目是“APEX2-based Proximity Labeling of Atox1 Identifies CRIP2 as a Nuclear Copper-binding Protein that Regulates Autophagy Activation”。本文通讯作者是来自北京大学药学院...
Enzyme‐catalyzed proximity labeling (PL) with the engineered ascorbate peroxidase APEX2 is a novel approach to map organelle compartmentalization and protein networks in living cells. Current procedures developed for mammalian cells do not allow delivery of the cosubstrate, biotin‐phenol, into living ...
近日,药学院天然药物及仿生药物国家重点实验室王晶团队在学术期刊《Angewandte Chemie International Edition》发表了题为“APEX2-based Proximity Labeling of Atox1 Identifies CRIP2 as a Nuclear Copper-binding Protein that Regulates Autophagy Activation”(利用APEX2邻近标记技术鉴定铜伴侣蛋白Atox1细胞核内铜结合蛋...
近日,药学院天然药物及仿生药物国家重点实验室王晶团队在学术期刊《Angewandte Chemie International Edition》发表了题为“APEX2-based Proximity Labeling of Atox1 Identifies CRIP2 as a Nuclear Copper-binding Protein that Regulates Autophagy Activation”(利用APEX2邻近标记技术鉴定铜伴侣蛋白Atox1细胞核内铜结合蛋...
Proximity labeling (PL) is an emerging technology for probing protein interactome in living cells. Current PL technology, like using engineered peroxidases (APEX2, HRP), is widely employed, benefiting from their high temporal resolution.1,2However, cytotoxicity of exogenous H2O2, which is used to ...
A genetically encoded peroxidase with improved sensitivity, APEX2, is reported for electron microscopy and proximity labeling at low expression levels. APEX is an engineered peroxidase that functions as an electron microscopy tag and a promiscuous labeli
近日,药学院天然药物及仿生药物国家重点实验室王晶团队在学术期刊《Angewandte Chemie International Edition》发表了题为"APEX2-based Proximity Labeling of Atox1 Identifies CRIP2 as a Nuclear Copper-binding Protein that Regulates Autophagy Activation"(利用APEX2邻近标记技术鉴定铜伴侣蛋白Atox1细胞核内铜结合蛋白...
In conclusion, we have employed the proximity-labeling enzyme APEX2 to identify 40 host cell proteins that interact with PEDV M protein. Co-IP confirmed the interactions in the case of five of these proteins, two of which (PPID and S100A11) down-regulated viral replication. Our data provide...
Enzymeヽatalyzed proximity labeling (PL) with the engineered ascorbate peroxidase APEX2 is a novel approach to map organelle compartmentalization and protein networks in living cells. Current procedures developed for mammalian cells do not allow delivery of the co﹕ubstrate, biotin﹑henol, into ...