Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat. Methods 2014, 12, 51-54. [CrossRef] [PubMed]Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. 2015. Directed evolution of APEX2 for electron microscopy and proximity labeling. ...
邻近蛋白(proximity labeling)生物素标记质粒通常指一种用于将生物素标签引入蛋白质附近的质粒。这种技术常用于研究蛋白质与其他生物分子(如蛋白质、RNA或DNA)之间的空间接近性和相互作用。 应用与优势 空间接近性研究:邻近蛋白生物素标记质粒可以用于研究蛋白质与其邻近分子(如相互作用伴侣或亚细胞结构)之间的接近性和交互...
Proximity labeling (PL) is an emerging technology for probing protein interactome in living cells. Current PL technology, like using engineered peroxidases (APEX2, HRP), is widely employed, benefiting from their high temporal resolution.1,2However, cytotoxicity of exogenous H2O2, which is used to ...
APEX is an engineered peroxidase that functions as an electron microscopy tag and a promiscuous labeling enzyme for live-cell proteomics. Because limited sensitivity precludes applications requiring low APEX expression, we used yeast-display evolution to improve its catalytic efficiency. APEX2 is far mor...
大家好,本周跟大家分享一篇发表在Angew上的文章,文章题目是“APEX2-based Proximity Labeling of Atox1 Identifies CRIP2 as a Nuclear Copper-binding Protein that Regulates Autophagy Activation”。本文通讯作者是来自北京大学药学院...
近日,药学院天然药物及仿生药物国家重点实验室王晶团队在学术期刊《Angewandte Chemie International Edition》发表了题为“APEX2-based Proximity Labeling of Atox1 Identifies CRIP2 as a Nuclear Copper-binding Protein that Regulates Autophagy Activation”(利用APEX2邻近标记技术鉴定铜伴侣蛋白Atox1细胞核内铜结合蛋...
近日,药学院天然药物及仿生药物国家重点实验室王晶团队在学术期刊《Angewandte Chemie International Edition》发表了题为“APEX2-based Proximity Labeling of Atox1 Identifies CRIP2 as a Nuclear Copper-binding Protein that Regulates Autophagy Activation”(利用APEX2邻近标记技术鉴定铜伴侣蛋白Atox1细胞核内铜结合蛋...
To address the need for an interaction-dependent proximity labeling tool, we describe here the development of an evolved split APEX2 (sAPEX) system. Using a novel yeast display- based directed evolution approach that incorporates positive and negative selection steps, we ...
Enzymeヽatalyzed proximity labeling (PL) with the engineered ascorbate peroxidase APEX2 is a novel approach to map organelle compartmentalization and protein networks in living cells. Current procedures developed for mammalian cells do not allow delivery of the co﹕ubstrate, biotin﹑henol, into ...
A genetically encoded peroxidase with improved sensitivity, APEX2, is reported for electron microscopy and proximity labeling at low expression levels. APEX is an engineered peroxidase that functions as an electron microscopy tag and a promiscuous labeli