subsequent experiments. We established the minimal boundary on labeling depth of APEX in acute slices using propargyl tyramide (PT), a clickable APEX substrate (Supplementary Fig.3c-d). Acute slices were incubated in ACSF supplemented with 250 µM PT for 1 h following the same protocol as...
Proximity labelingLipid droplets (LDs) are ubiquitous lipid storage organelles composed of a neutral lipid core surrounded by a phospholipid monolayer that is decorated with integral and peripheral proteins. Accurate...doi:10.1007/978-1-4939-9537-0_5Kirill Bersuker...
Transfections were performed as described in the protocol. Briefly, cells were seeded to different plates, and the next day, the DNA and Lipofectamine were mixed and added to the cells. Forty hours later, expression was detected by western blot or immunofluorescence. For imaging experiments, ...
providing a tool to visualize these subcellular structures with high contrast and with the resolution of an electron microscope. In the presence of H2O2, APEX2 catalyzes the polymerization of DAB, generating a brown precipitate that can be visualized in ...