This task remains challenging in complex tissue, due to suboptimal protein isolation techniques that often result in loss of cell-type specific information and incomplete capture of subcellular compartments. Here, we develop a genetically targeted proximity labeling approach to identify cell-type specific...
Combined with stable isotope labeling by amino acids in cell culture (SILAC), we demonstrate proteomic mapping of a membrane‐enclosed and a semiopen compartment, the mitochondrial matrix and the nucleus. APEX2 PL revealed nuclear proteins that were previously not identified by conventional techniques...
Furthermore, techniques that provide adequate temporal information often focus on one pathway at a time, requiring a large array of assays. The use of APEX2, a proximity-dependent labeling technique, provides both spatial and temporal information in endogenous protein expression level, allowing for ...
To overcome the limitations of traditional methods, proximity labeling techniques like APEX2 have been developed. Unlike proximity-dependent biotin identifi- cation (BioID), which requires extended labeling times [14,15], or the antibody-dependent SPLAAT and EMARS methods [16,17], APEX2 enables ...