An electrophoretic mobility shift assay was used to examine the binding of transcription factors to the cyclin D1 geneAP-1 motif.The results showed that the proliferation of PG cells treated with different concentrations (10, 20, and 40 渭g/mL) of berberine for 24 and 48 h was suppressed ...
然后,作者通过流式细胞术分选出eGFP + 细胞,并进行ATAC-seq和转录因子足迹分析。与体外结果一致(图5a),作者发现AP-1家族成员是PD-1缺陷细胞中富集的顶级motif(图5g)。令人惊讶的是,在ACLY药物抑制的情况下,与完整的PD-1通路相比,Pdcd1缺失细胞中的AP-1足迹被减少(图5h)。 总的来说,作者基因定义的小鼠模型...
Also interesting is the fact that the baseline expression of LDLR is also inhibited by the mutations, suggesting that the AP-1 motif may be important for the basal expression of the receptor as well. Given that the culture medium contains 10% fetal bovine serum, and the c-fos expression ...
Berberine inhibits cyclin D1 expression via suppressed binding of AP-1 transcription factors to CCND1 AP-1 motif. Acta Pharmacol Sin. 29(5):628-33(2008). [5] Gaudio E, et al. Physical association with WWOX suppresses c-Jun transcriptional activity.Cancer Res. 66(24):11585-9 (2006). ...
Motif logos are shown. Black, dotted boxes highlight core AP-1 TF-motif. Note that transcriptional repressor BACH shares AP1-motif. Data in (b-i) are collected from 2 biologically independent experiments. j, ATAC-seq (grey lines-forward, black lines-reverse reads) and nucleosome density (...
The transporter carries an NH2-terminal endosomal/lysosomal targeting motif ([DE]XXXL[LI]). Accordingly, constitutive GLUT8 has been found to be associated... S Schmidt,HG Joost,A Schürmann - 《Am J Physiol Endocrinol Metab》 被引量: 141发表: 2009年 A biochemical analysis linking APOBEC3A ...
AP-1motiffortheconstitutiveandphorbolester——inducibleex?? pressionoftheurokinase-typeplasminogenactivatorreceptor gene[J].JBiolChem,1996,271(38):231764 [14]KuniyasuH,ChiharaY,KubozoeT,eta1.Co—expressionof CD44v3andheparanaseiscorrelatedwithmetastasisofhuman ...
MEME-ChIP: motif analysis of large DNA datasets. Bioinformatics 2011; 27: 1696–1697. Article CAS PubMed PubMed Central Google Scholar Download references Acknowledgements We thank Dr Fan Yang (NCI/NIH), Dr Kairong Cui (NHLBI/NIH), Dr Bingmei Zhu (NIDDK/NIH), Jeffery Burnett (NIDCD/NIH...
3.2. ICC-specific super enhancers affect gene expression in ICC and the region of different histone modification patterns are enrichment in sites of AP-1 motif As shown inFig. 2, we first identify Super-Enhancers (SEs) using H3K27ac in the genomes of HIBEpiC, SSP-25, HUCCT1, CCLP-1, ...
To identify BATF-containing TF complexes in ALCL, we first analyzed AP-1 DNA binding at the classical AP-1 5ʹ-TGA[G/C]TCA-3ʹ motif (Supplementary Figure1A, top) in a panel of ALK+and ALK–ALCL and T-cell-derived control (non-ALCL) cell lines already used in our previous stud...