An i-motif-containing DNA device is constructed that is able to break certain forms of Watson–Crick interactions under isothermal conditions. Y Wang,X Li,X Liu,... - 《Chemical Communications》 被引量: 21发表: 2007年 An i-motif-containing DNA device that breaks certain forms of Watson-cri...
在含有YAR motif的靶点中,AXBE可产生15-32%的A到Y碱基颠换编辑。AXBE在HeLa细胞系中也可达到高达46%的腺嘌呤碱基颠换。通过Cas9依赖性/非依赖性脱靶评估以及RNA-seq脱靶检测发现,相比于ABE系统,AXBE的脱靶明显降低,尤其是在RNA水平上降低了90%。 为增加腺嘌呤颠换编辑效率并拓展靶向范围,基于结构导向的理性设计和...
The RNA Recognition Motif of Eukaryotic Translation Initiation Factor 3g (eIF3g) Is Required for Resumption of Scanning of Posttermination Ribosomes for Re... The RNA recognition motif of eukaryotic translation initiation factor 3g (eIF3g) is required for resumption of scanning of posttermination rib...
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为了验证鉴定的motif,作者进行了TF足迹分析,证实了TF与DNA的结合。作者发现这些基序在相应的细胞类型中表现出活跃的TF结合(图5)。总之,作者生成了斑马鱼早期胚胎发生的染色质可及性图谱,证明了与相应的已发表的scRNA-seq数据的高度一致性。 图5. 足迹分析确定了snATAC-seq数据中具有代表性的细胞类型特定的TF活动。
Cloning of cDNAs coding for human HMG I and HMG Y proteins: both are capable of binding to the octamer sequence motif In human B lymphocytes and placenta HMG I and its smaller isoform HMG Y are encoded by two distinct but structurally highly similar mRNAs which arise most ... E Richard,...
12.Kagale S,Links M G,Rozwadowski K.Genome-wide analysis of ethylene-responsive element binding factor-associated amphiphilic repression motif-containing transcriptional regulators inArabidopsis[J].Plant Physiology,2010,152(3):1109-1134. 13.Gong...
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Genetic mutation or autoantibody inhibition of the ultra-large von-Willebrand factor protease, A Disintegrin And Metalloproteinase with a Thrombospondin Type 1 Motif (ADAMTS13), reduces its enzymatic activity and is an important factor that promotes TTP development. Case reports of acute TTP episodes ...