conformational changemacrolide antibiotic binding protein (MABP)non-canonical ABC transporterMacrolide antibioticErythromycinRv3197 (MABP-1), a non-canonical ABC protein in Mycobacterium tuberculosis, has ATPase activity and confers inducible resistance to the macrolide family of antibiot...
The binding of recombinant p8 protein to double-stranded DNA was inhibited by biotinylated-clarithromycin, clarithromycin, erythromycin and azithromycin in gel mobility shift assay. Dechlorogriseofulvin, obtained from a natural product screening, also inhibited human p8 protein binding to DNA. This study...
The drug-binding protein AlbA confers albicidin resistance to Klebsiella oxytoca. Here we show that AlbA binds albicidin with low nanomolar affinity resulting in full inhibition of its antibacterial activity. We report on the crystal structure of the drug-binding domain of AlbA (AlbAS) in ...
Lysophosphatidic acid-induced c-fos up-regulation involves cyclic AMP response element-binding protein activated by mitogen- and stress-activated protein k... We report rapid-freeze-quench X-ray absorption spectroscopy of a dizinc metallo-beta-lactamase (MBL) reaction intermediate. The Zn(II) ions...
ß-Lactam Antibiotic-Induced Release of Free Endotoxin: In Vitro Comparison of Penicillin-Binding Protein (PBP) 2-Specific Imipenem and PBP 3-Specific Ce... The relative effects of two β-lactam antibiotics, penicillin-binding protein (PBP) 2-specific imipenem and PBP 3-specific ceftazidime, ...
7d,e). Next, we constructed RifHS mutants in cells containing a temperature-sensitive allele of the replication protein dnaC2 (PC2)39,42. We verified that shifting cells to the non-permissive temperature 42 °C inhibits replication (Extended Data Fig. 7f). We then compared the bactericidal...
The yfdX family proteins are known for long time to occur in various virulent bacteria including their multidrug resistant (MDR) strains, without any direct assigned function for them. However, yfdX protein along with other proteins involved in acid tole
"Using cryo-electron microscopy, it was possible to determine the binding site of tetracycinomycin X to the ribosome, it is located in the tunnel through which the synthesized peptide leaves the ribosome, opposite the site in which the well-known protein synthesis inhibitors—macrolides and step...
The understanding of protein—ligand binding is of critical importance for biomedical research, yet the process itself has been very difficult to study bec... I Buch,T Giorgino,GD Fabritiis - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 391发表...
Antibiotics are widely used in daily life but their abuse has posed a potential threat to human health. To evaluate the toxicity of chloramphenicol (CAP) at the protein level, the interaction between CAP and human serum albumin (HSA) was investigated by fluorescence, Ultraviolet-visible (UV-Vis...