An aminoacylation/translation (AIT) system based on the protein synthesis system from the pathogen , was used to screen chemical compounds for identifying inhibitors of protein synthesis. This system includes elongation factors: EF-Tu, EF-Ts and EF-G, aminoacyl tRNA synthetase (aaRS) specific for...
Answer to: Which would be better: an antibiotic that prevents the synthesis of flagellin (protein in flagella) or an antibiotic that prevents...
Environmental changes influence the susceptibility ofSalmonellaandShigellato CTR that targets cell wall inhibition as seen from the increase in sensitivity to the antibiotic during PF in non-affected locations. Increase in sensitivity to CTR as seen inSalmonella(typhoid) andShigella(shigellosis-acute blood...
Other antibiotics, such as theaminoglycosides, chloramphenicol,erythromycin, and clindamycin, inhibitproteinsynthesis in bacteria. The basic process by which bacteria andanimalcells synthesize proteins is similar, but the proteins involved are different. Those antibiotics that are selectively toxic utilize the...
3c). Taken together, the convergent and colocalized resistance mutations in lptF along with biochemical data provide strong evidence that zosurabalpin targets the inner-membrane LptB2FGC complex to block LPS transport. Fig. 3: Zosurabalpin kills cells by inhibiting LptB2FGC function. a, ...
Combined, our findings signify an important step toward unraveling shared genetic mechanisms underlying bacterial persistence and, therefore, identifying primary persistence therapy targets. Results Cellular Obg Levels Control Persistence in E. coli Obg is at the nexus of DNA and protein synthesis. ...
AA4 to produce amycomicin, a new antibiotic that targets fatty acid biosynthesis and kills S. aureus in both in vitro and in vivo assays with submicromolar activity (MIC 30 nM) (Figure 2c). Subsequently, they found that alteration of the carbon source by S. coelicolor metabolism induces ...
Importantly, since this compound class is completely new and the protein LpxH has not yet been exploited as a target for antibiotics there is no pre-existing resistance to this class of compounds. This is in contrast to the many ‘me-too’ antibiotics of existing classes currently in clinical...
S. Global transcriptional response of Bacillus subtilis to treatment with subinhibitory concentrations of antibiotics that inhibit protein synthesis. Antimicrob. Agents Chemother. 49, 1915–1926 (2005). CAS PubMed PubMed Central Google Scholar Wu, X. et al. Dynamic proteome response of Pseudomonas ...
Through direct interaction with EF-G, FusB promotes disassembly of the FA-stalled post-translocation complex, thereby rescuing protein synthesis14,16. FusB has also been shown to increase turnover of EF-G on the ribosome in the absence of FA, 1School of Molecular and Cellular Biology, Gars...