Finally, linkage-specific polyubiquitin antibodies revealed that K48-linked ubiquitin chains localize to rhTRIM5伪 cytoplasmic bodies, implicating these structures in proteasomal degradation. Differential staining of cytoplasmic bodies seen with different polyubiquitin antibodies suggests that structural changes ...
Polyubiquitination can be characterized by Lys (K)48-, K63-, and other linkages of ubiquitin chains based on which lysine residue of ubiquitin is bound to a growing ubiquitin chain [29]. Next, we examined Sigirr ubiquitination with a linkage-specific polyubiquitin pulldown and revealed that the...
The K48-linkage-specific antibody also specifically reacted with Bcl- B carrying 2, 3, 4 or more ubiquitins, but not—as expected—with monoubiquitinated Bcl-B (lanes 7, 8). This confirmed the presence of K48-linked polyubiquitin on Bcl-B. The K63-linkage-specific antibody detected some...
5A), supporting the potential AR-E2 ubiquitin-conjugating function of UBE2J1. Considering that the most common pattern of ubiquitination associated with protein degradation is lysine 48 (K48)-linked ubiquitination [34,35,36], we conducted a co-IP of AR in wildtype LNCaP/AR and UBE2J1-KO ...
Anti-Ubiquitin K48-Specific Antibody, clone Apu2 ZooMAb® Rabbit Monoclonal View Price and Availability Sigma-Aldrich SAB4200745 Anti-Protein A antibody, Mouse Monoclonal View Price and Availability Sigma-Aldrich R0278 RIPA Buffer View Price and Availability Sigma-Aldrich SAB1402914 Monoclonal Anti-SF...
One particular polyubiquitin tag is the K48-linked ubiquitin chain that addresses the tagged protein for proteasomal degradation. Conveniently, this pathway is exploited by pathogens to degrade key components of the innate immune system, particularly those within the NF-κB pathway (Table S1). For ...
Using the K29-ubiquitin chain-specific binder sAB-K2946, we show that Aurora B and Survivin are modified by this atypical ubiquitin chain and that TRABID is capable of removing such modification from the two proteins. Interestingly, while our study proposes a role of TRABID-mediated K29-deubi...
K63R or K48R represented the corresponding lysine was replaced by arginine on ubiquitin, respectively. q Binding of endogenous SIRT7 to UBR5 was assessed in cells with or without GSK3β knockdown. r Binding of SIRT7-WT and the indicated mutants with UBR5 was evaluated in HEK293 cells. ...
(Fig.6b). We then used introduced ubiquitin K48 or K63 mutants where all Lys except K48 or K63 were mutated to Arg. TRAF3IP3 specifically increased K48-linked but not K63-linked polyubiquitination of TBK1 (Fig.6c), suggesting that TRAF3IP3 increased the degradative ubiquitination of TBK1...
to theActbmRNA level.iWT and FAM73b KO BMDMs were stimulated with LPS for 60 min and incubated with MG132 for 30 min. IRF1 was isolated by IP (under denaturing conditions), and its ubiquitinated form was detected by IB using anti-K48-linked ubiquitin antibody. Protein lysates were ...