The primary antisera used included mouse polyclonal antiserum against TNF-α (1;1000, Abcam, UK), IL-1β (1;1000, Abcam, UK), IL-6 (1;1000, Abcam, UK), Iba-1 (1;1000, Wako, Japan) and CD11b (1;1000, Abcam, UK), γ-H2AX (1:500, Abcam, UK), UNG (1:1000; Abcam, UK...
1% of the sample was saved as input, and the rest was incubated with 5 µg Anti-AR Antibody (Abcam, ab108341) or 5 µg Normal Rabbit IgG Polyclonal Antibody (Millipore Sigma, 12-370) overnight at 4 °C. After overnight incubation, the samples were incubated for 4 h at...
The sections were incubated with anti-HMGB1 mouse monoclonal antibody (R&D Systems, Minneapolis, MN, USA), anti-alpha smooth muscle actin (α- SMA) rabbit polyclonal antibody (Abcam, Cambridge, UK) or anti-Iba1 rabbit polyclonal antibody (Wako, Osaka, Japan). The sections were also ...
CPXM2 羧肽酶X(M14家族2)抗体 规格: 0.2ml 桑皮苷A(标准品) Mulberroside A phospho-C CBL(Tyr674) 化原基因CBL2抗体 规格: 0.1ml 代森锌 Zineb 氯化钠多粘菌素B肉汤基础(SCPB) 250(g) HA(204-212) Influenza HA (204 - 212) 小鼠神经型一化氮合酶(NOS1)ELISA试剂盒 英文名: NOS1 ELISA Kit...
IHC was performed using formalin-fixed, paraffin-embedded (FFPE) tumors sectioned at a thickness of 5 μm with monoclonal anti-CD31 antibody (Abcam, Cambridge, UK), polyclonal anti-F4/80 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and polyclonal anti-VEGF antibody (Santa Cruz ...
Rabbit polyclonal antibodies against HLA Class II DRB1 (HLA-DRB1) (1:2000, ab133578, Abcam, UK) and HLA Class I A (HLA-A) (1:400, ab52922, Abcam, UK) were used as the primary antibodies. IHC assays for HLA-A and HLA-DRB1 were performed by RecordBio (Shanghai, China). Sections...
Caspase 4/p20/p10 Polyclonal antibody (#11,856–1-AP) was from Proteintech. ASC/TMS1/PYCARD Antibody (B-3) (#sc-514,414) was from Santa Cruz. Dimethyl Sulfoxide (DMSO) (#D2650–100ml) was purchased from Sigma. Sodium carboxymethylcellulose (CMC) was from Shanghai Yuanye Bio-Technology...
and then incubated with rabbit polyclonal anti-LC3B antibody (1:200 dilution) for 1 h. After extensive washing with DPBS, the cells were incubated with Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:300 dilution, Invitrogen) for 1 h. The cells were counterstained with DAPI and obse...
www.nature.com/scientificreports OPEN Anti-high mobility group box-1 (HMGB1) antibody inhibits hemorrhage-induced brain injury received: 28 November 2016 accepted: 09 March 2017 Published: 10 April 2017 and improved neurological deficits in rats Dengli Wang1, Keyue Liu1, Hidenori Wake1, ...
Following incubation, cells were pelleted and washed two times in 200 µL assay buffer before the addition of secondary antibody: rabbit polyclonal antibody to human albumin (Abcam, #ab2406). Plates were incubated and the wash routine was repeated before the tertiary antibody, goat anti-...