IHC was performed using formalin-fixed, paraffin-embedded (FFPE) tumors sectioned at a thickness of 5 μm with monoclonal anti-CD31 antibody (Abcam, Cambridge, UK), polyclonal anti-F4/80 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and polyclonal anti-VEGF antibody (Santa Cruz ...
1% of the sample was saved as input, and the rest was incubated with 5 µg Anti-AR Antibody (Abcam, ab108341) or 5 µg Normal Rabbit IgG Polyclonal Antibody (Millipore Sigma, 12-370) overnight at 4 °C. After overnight incubation, the samples were incubated for 4 h at...
www.nature.com/scientificreports OPEN Anti-high mobility group box-1 (HMGB1) antibody inhibits hemorrhage-induced brain injury received: 28 November 2016 accepted: 09 March 2017 Published: 10 April 2017 and improved neurological deficits in rats Dengli Wang1, Keyue Liu1, Hidenori Wake1, ...
The orally active quinoline-3-carboxamide tasquinimod [ABR-215050; CAS number 254964-60-8), which currently is in a phase II-clinical trial in patients against metastatic prostate cancer, exhibits anti-tumor activity via inhibition of tumor angiogenesis
The primary antibodies used for Western blot analysis were rabbit polyclonal Smad2/3 antibody (sc-8332; Santa Cruz Biotechnology), rabbit phospho-Smad3 antibody (9520; Cell Signaling Technology, Danvers, MA, USA), goat polyclonal BCAR3 antibody (sc-47811; Santa Cruz Biotechnology), rabbit polyclon...
One approach that has been explored is the development of Treg cell–based therapy based on the adoptive transfer of polyclonal Treg cells into patients with autoimmunity, in which may lead to the consequences of antigen-nonspecifically suppressing host defense and T cell immunity [12]. Therefore,...
The sections were incubated with anti-HMGB1 mouse monoclonal antibody (R&D Systems, Minneapolis, MN, USA), anti-alpha smooth muscle actin (α- SMA) rabbit polyclonal antibody (Abcam, Cambridge, UK) or anti-Iba1 rabbit polyclonal antibody (Wako, Osaka, Japan). The sections were also ...
The separated proteins were transferred to a 0.45-μm nitrocellulose membrane (Bio-Rad, USA) and probed using Anti-MUC1-CD (AB2) rabbit polyclonal antibody (catalogue code AV41446, Sigma-Aldrich, USA) diluted 1:250 and Anti-β-actin mouse monoclonal antibody diluted 1:5000. Western ...
How cells decipher the duration of an external signal into different transcriptional outcomes is poorly understood. The hormone gastrin can promote a variety of cellular responses including proliferation, differentiation, migration and anti-apoptosis. Wh
Mifepristone (MF) has been largely used in reproductive medicine due to its capacity to modulate the progesterone receptor (PR). The study of MF has been expanded to the field of oncology; yet it remains unclear whether the expression of PR is required f