After washing, the slices were incubated with rabbit anti-NeuN (1: 500, Millipore) in 10% goat serum PBS buffer followed by a red fluorescent-conjugated secondary antibody (Thermo Fisher Scientific Inc.) for another 1 h. DAPI was used as the blue nuclear stain. The expression and ...
1% of the sample was saved as input, and the rest was incubated with 5 µg Anti-AR Antibody (Abcam, ab108341) or 5 µg Normal Rabbit IgG Polyclonal Antibody (Millipore Sigma, 12-370) overnight at 4 °C. After overnight incubation, the samples were incubated for 4 h at...
The separated proteins were transferred to a 0.45-μm nitrocellulose membrane (Bio-Rad, USA) and probed using Anti-MUC1-CD (AB2) rabbit polyclonal antibody (catalogue code AV41446, Sigma-Aldrich, USA) diluted 1:250 and Anti-β-actin mouse monoclonal antibody diluted 1:5000. Western blot ...
www.nature.com/scientificreports OPEN Anti-high mobility group box-1 (HMGB1) antibody inhibits hemorrhage-induced brain injury received: 28 November 2016 accepted: 09 March 2017 Published: 10 April 2017 and improved neurological deficits in rats Dengli Wang1, Keyue Liu1, Hidenori Wake1, ...
Following incubation, cells were pelleted and washed two times in 200 µL assay buffer before the addition of secondary antibody: rabbit polyclonal antibody to human albumin (Abcam, #ab2406). Plates were incubated and the wash routine was repeated before the tertiary antibody, goat anti-...
Mifepristone (MF) has been largely used in reproductive medicine due to its capacity to modulate the progesterone receptor (PR). The study of MF has been expanded to the field of oncology; yet it remains unclear whether the expression of PR is required f
We used two well-known polyvalent antisera against IgG, IgM, and C3 generated in either goat or rabbit, and achieved very similar results by the MICRO, CAPIL, and GEL LAB DAT methods in this study. However, for FLOW DAT, the polyclonal antiglobulins appeared to produce nonspecific irreproduci...
www.nature.com/scientificreports OPEN received: 13 June 2016 accepted: 13 October 2016 Published: 08 November 2016 Peripheral and spinal 5-HT receptors participate in cholestatic itch and antinociception induced by bile duct ligation in rats Bin Tian1,*, Xue-Long Wang1,*,†,Ya Huang2...
and then incubated with rabbit polyclonal anti-LC3B antibody (1:200 dilution) for 1 h. After extensive washing with DPBS, the cells were incubated with Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:300 dilution, Invitrogen) for 1 h. The cells were counterstained with DAPI and obse...
Cells were then incubated with the following primary antibodies: glucocorticoid receptor (D6H2L) (1:200) (Cell Signaling), phospho-glucocorticoid receptor (Ser211) (1:2000) (Cell Signaling), anti-NF-κB p65 antibody [E379] (1:200) (Abcam), and anti-Ki67 polyclonal antibody (1:400) (...