Decreased heart rate variability (HRV) leads to cardiovascular diseases and increased mortality in clinical studies. However, the underlying mechanisms are still inconclusive. Systemic inflammation-induced neuroinflammation is known to impair the autonom
The primary antibodies used for Western blot analysis were rabbit polyclonal Smad2/3 antibody (sc-8332; Santa Cruz Biotechnology), rabbit phospho-Smad3 antibody (9520; Cell Signaling Technology, Danvers, MA, USA), goat polyclonal BCAR3 antibody (sc-47811; Santa Cruz Biotechnology), rabbit polyclon...
1% of the sample was saved as input, and the rest was incubated with 5 µg Anti-AR Antibody (Abcam, ab108341) or 5 µg Normal Rabbit IgG Polyclonal Antibody (Millipore Sigma, 12-370) overnight at 4 °C. After overnight incubation, the samples were incubated for 4 h at...
An aliquot (100 μg of protein) from each NP-40 cell lysate was incubated overnight at 4°C with constant rotation in 1 ml of NP-40 lysis buffer containing 1 μg polyclonal rabbit antibody to Cdk2 (M2; #sc-163; Santa Cruz Biotechnology). Immunocomplexes associated with Cdk2 were colle...
www.nature.com/scientificreports OPEN Anti-high mobility group box-1 (HMGB1) antibody inhibits hemorrhage-induced brain injury received: 28 November 2016 accepted: 09 March 2017 Published: 10 April 2017 and improved neurological deficits in rats Dengli Wang1, Keyue Liu1, Hidenori Wake1, ...
(Santa Cruz Biotechnology, Santa Cruz, CA, USA) and polyclonal anti-VEGF antibody (Santa Cruz Biotechnology). CD31, F4/80 and VEGF staining represented microvessel, macrophage and VEGF protein density, respectively. Staining and imaging were performed as described previously32,33. Tumor section ...
As a control, because the TSP1 antibody (rabbit polyclonal Ab8) cross-reacts with TSP1 of mouse origin, mRNA levels of mouse TSP1 was also measured, to assure that host derived TSP1 was not the source of increased TSP1 production in the treated tumors via infiltrating cells and blood (...
Caspase 4/p20/p10 Polyclonal antibody (#11,856–1-AP) was from Proteintech. ASC/TMS1/PYCARD Antibody (B-3) (#sc-514,414) was from Santa Cruz. Dimethyl Sulfoxide (DMSO) (#D2650–100ml) was purchased from Sigma. Sodium carboxymethylcellulose (CMC) was from Shanghai Yuanye Bio-Technology...
The following antibodies were used: monoclonal (mouse) anti-human α-Tubulin (reacts with mouse, rat and human) (1:300; sc-5286, Santa Cruz Biotechnology), polyclonal horseradish peroxidase (HRP) conjugated (goat) anti-mouse (1:3000; PO447, DAKO), polyclonal (rabbit) phospho-p44/42 MAPK...
and then incubated with rabbit polyclonal anti-LC3B antibody (1:200 dilution) for 1 h. After extensive washing with DPBS, the cells were incubated with Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:300 dilution, Invitrogen) for 1 h. The cells were counterstained with DAPI and obse...